肝豆状核变性患者尿铜及血清铜的测定对临床诊断的意义

Clinical diagnosis significance of urine copper and serum copper determination on patients with hepatolenticular degeneration

目的：探讨肝豆状核变性与尿铜和血清铜的相关性及其对临床诊断及治疗的意义。方法在血清样品和尿样中加入一定量的硝酸溶液使其酸度为1%，石墨炉原子吸收光谱法测定其中的铜含量。结果尿铜和血清铜对于肝豆状核变性均不具有单独的诊断价值，但他们确是诊断肝豆状核变性的重要参考指标。该方法检出限为1.0μg/L，相对标准偏差为2.23%，方法实测加标回收率为94%~104%。结论尿铜及血清铜检测已广泛应用于临床样本的测定，对指导肝豆状核变性患者的康复有良好的辅助作用。

Objective To investigate the relationship between urine copper and serum copper in patients with hepatolenticular degeneration and their significance on clinical diagnosis and treatment. Methods A method for the determination of copper in urine and serum in hepatolenticular degeneration patient was developed,take 1% of nitric acid to nitrify the serum and urine,and use Graphite furnace a-tomic absorption to detect. Results The results showed that urine copper and serum copper contents were really important reference index for diagnosing the disease,although they did not have separate diag-nosing significance. The detection limit of copper was 1. 00 μg/L,the relative standard deviation was 2. 23%. The recovery rates was 94% -104%. Conclusions Determination of urine copper and serum copper has been applied to detect the clinical samples and played a good role for the hepatolenticular de-generation patient’s rehabilitation.