摘要
Marine zooplankton responds sensitively to elevated seawater CO_2 concentration. However, the underlying physiological mechanisms have not been studied well. We therefore investigated the effects of elevated CO_2concentration（0.08%, 0.20%, 0.50% and 1.00%） on antioxidant defense components, as well as two detoxification enzymes of Calanus sinicus（copepod）. The results showed that glutathione peroxidase（GPx） activity exposed to CO_2-acidified seawater was significantly stimulated while other antioxidant components, including glutathione-Stransferase（GST） activity, superoxide dismutase（SOD） activity decreased significantly with reduced glutathione（GSH） level and GSH/oxidized glutathione（GSSG） value. CO_2-acidified seawater exhibited stimulatory effects on adenosine triphosphatase（ATPase） activity and acetylcholinesterase（Ach E） activity was inhibited. Moreover, the results of principal component analysis indicated that 75.93% of the overall variance was explained by the first two principal components. The elevated CO_2 concentration may affect the metabolism and survivals of copepods through impacts these enzymes activities. Further studies are needed to focus on the synergistic effects of elevated CO_2 concentration and other environmental factors on copepods.
Marine zooplankton responds sensitively to elevated seawater CO_2 concentration. However, the underlying physiological mechanisms have not been studied well. We therefore investigated the effects of elevated CO_2concentration（0.08%, 0.20%, 0.50% and 1.00%） on antioxidant defense components, as well as two detoxification enzymes of Calanus sinicus（copepod）. The results showed that glutathione peroxidase（GPx） activity exposed to CO_2-acidified seawater was significantly stimulated while other antioxidant components, including glutathione-Stransferase（GST） activity, superoxide dismutase（SOD） activity decreased significantly with reduced glutathione（GSH） level and GSH/oxidized glutathione（GSSG） value. CO_2-acidified seawater exhibited stimulatory effects on adenosine triphosphatase（ATPase） activity and acetylcholinesterase（Ach E） activity was inhibited. Moreover, the results of principal component analysis indicated that 75.93% of the overall variance was explained by the first two principal components. The elevated CO_2 concentration may affect the metabolism and survivals of copepods through impacts these enzymes activities. Further studies are needed to focus on the synergistic effects of elevated CO_2 concentration and other environmental factors on copepods.
