养血清脑颗粒对脑缺血再灌注大鼠促血管生长因子的影响

Effects of Yangxueqingnao granule on the expression of vascular growth factors in rats with focal cerebral ischemia-reperfusion

目的研究养血清脑颗粒对大鼠脑缺血再灌注损伤后脑组织中碱性成纤维细胞生长因子(b FGF)和血管内皮生长因子(VEGF)表达的影响。方法将SD大鼠随机分为假手术组、模型组和实验组,每组8只。模型组和实验组用线栓法制作大鼠大脑中动脉脑缺血再灌注模型。实验组于再灌注3 h后灌胃给予养血清脑颗粒1 g·kg^(-1),qd,连续给药7 d。假手术组和模型组于再灌注3 h后灌胃给予相同体积的灭菌0.9%Na Cl溶液。用免疫组织化学法和实时定量聚合酶链式反应法检测缺血区脑组织中b FGF和VEGF的表达。结果模型组、假手术组、实验组大鼠脑组织中b FGF阳性细胞数分别为(23.92±3.84),(3.78±0.58),(34.08±5.64);VEGF阳性细胞数分别为(26.87±4.56),(5.24±0.67),(37.45±6.29),模型组的b FGF和VEGF阳性细胞数与假手术组比较差异有统计学意义(P<0.05),实验组的b FGF和VEGF阳性细胞数与模型组比较差异有统计学意义(P<0.05)。结论养血清脑颗粒可通过促进b FGF和VEGF的表达,从而发挥对脑缺血再灌注损伤的神经保护作用。

Objective To investigate the effects of Yangxueqignao granule on the expression of basic fibroblast growth factor （bFGF） and vascular endothelial growth factor （VEGF） in rats with focal cerebral ischemia- reperfusion. Methods Rats were randomly assigned into three groups：sham operation group（ sham group） , focal cerebral ischemia -reperfusion group （model group ） and Yangxueqingnao granule group （test group）. The middle cerebral artery occlusion reperfusion model was made by the suture method. The rats in the test group were lavaged with Yangxueqignao granule 1 g· kg^-1 after making a model, the rats in the sham group and model group were injected with the same dosage of normal saline. The levels of bFGF and VEGF expression were measured by immunohistoehemistry and real time polymerase chain reaction at one week after ischemia - reperfusion. Results Among the model, sham, and test groups, the number of bFGF positive cells in rats＇ brain tissue were （23.92 ±3.84）, （3.78±0. 58）, （34. 08±5.64）, and the number of VEGF positive cells were （ 26. 87 ± 4. 56 ） , （ 5.24 ± 0. 67 ）, （37.45 ± 6.29 ）. There were significant differences in the number of bFGF positive cells as well as VEGF positive cells between model and sham group （ P 〈 0.05 ）. Similar results were also found between model and test group （P 〈 O. 05 ）. Conclusion Yangxueqingnao granule can protect cerebral ischemia injury partly by enhancing the expressions of bFGF and VEGF in brain.