miRNA-194对人骨肉瘤细胞系SOSP-9607转移的作用研究

Effects of MiR-194 on Metastasis of Human Osteosarcoma Cell Line SOSP-9607

目的:通过建立表达不同高低水平miRNA-194的骨肉瘤细胞系,研究和探测miRNA-194对于骨肉瘤细胞转移特性的影响和作用。为进一步研究miRNA-194作为生物治疗的新靶点提供理论依据。方法:使用慢病毒技术,对骨肉瘤细胞进行转染和筛选,获得表达不同高低水平miRNA-194的骨肉瘤细胞系并进行分组。通过Transwell实验,划痕实验对miRNA-194在骨肉瘤中的作用进行探索。结果:1)慢病毒转染及筛选成功,获得表达不同高低水平miRNA-194的骨肉瘤细胞系;2)transwell迁移及侵袭实验中,miRNA-194过表达组的迁移(28.60±4.36)及侵袭(21.25±6.42)能力都显著的小于其余各组,相应的,miRNA-194沉默表达组(132.60±15.64;115.76±11.38)则高于其余各组。划痕实验结果显示,miRNA能够显著的抑制骨肉瘤细胞SOSP-9607的划痕愈合能力(P<0.01)。结论:miR-194能够对骨肉瘤细胞SOSP-9607的转移起到明确的抑制作用。MiRNA-194有望成为骨肉瘤转移与治疗的新靶点。

Objective： miRNA-194 were knocked down or overexpressed in Osteosarcoma Cell Line SOSP-9607 to investigate its effect on Metastasis of human Osteosarcoma which to provide the foundation of theory of miRNA-194 as a new target of osteosarcoma therapy. Methods： We transfected human osteosarcoma cell lines SOSP-9607 by using recombinant lentiviruses and divided the cells into three groups with different levels of miRNA-194. Then the stable transfected cells were used in transwell and wound healing assay.Results： 1） The transfection and selection of miRNA-194 using recombinant lentiviral expression vector was finished correctly and different levels of mi RNA-194 were got sucessfully. 2） Results in the transwell migration and invasion assay showed significantly lower numbers of OE SOSP-9607 cells（28.60±4.36） and（21.25±6.42） and significantly higher numbers of KD SOSP-9607 cells（132.60±15.64;115.76±11.38） compared with other groups（p〈0.05）. The wound healing assay showed that cells in OE groups exhibited a significant decrease in migration rate compared to the other three groups（p〈0.05）. Conclusions： miRNA-194 could inhibit metastasis of Osteosarcoma Cell Line SOSP-9607. MiRNA-194 could be further explored as a potential target in Osteosarcoma diagnosis and therapy.