利用AFLP标记对鄂西多花木蓝材料遗传变异的分析

Analysis of Genetic Variation of Indigofera amblyantha Craib cv. Exi Based on AFLP

利用AFLP标记研究了鄂西多花木蓝在贵州种植近10年的栽培材料、辐射诱变材料及野生材料共36份材料间的遗传变异情况,结果表明:8对AFLP引物共扩增出926条带,平均扩增115.8条带,多态性条带共609条,平均多态性条带76.1条,多态性条带占65.8%,表明各参试材料间出现了较大的遗传变异;材料样本间平均相似系数为0.774,栽培材料的平均GS为0.778,野生材料的平均GS为0.742,C10辐射诱变材料的平均GS为0.824,C2辐射诱变材料的平均GS为0.791,说明野生材料的遗传变异最大,辐射诱变材料变异最小;聚类分析表明,材料可分为6大类,栽培材料聚类比较集中,辐射带来的变异具有很大的不确定性,其聚类较为分散;贵州高原复杂的地理环境对野生多花木蓝遗传分化影响较大,野生材料聚类受区域性小环境的影响较大。

Using AFLP marker,the genetic variation of Indigofera amblyantha cv.Exi cultivated for10 years in Guizhou,radiation induced Indigofera amblyantha and wild Indigofera amblyantha were studied,the results showed that：1）8pairs AFLP markers generated 926 bands,115.8bands were amplified in average,and the number of polymorphic bands were 609,65.8% were polymorphic bands.There was rich genetic diversity among the tested materials of Indigofera amblyantha.2）The mean similarity coefficient was 0.774 among the tested materials,the similarity coefficient of the cultivated materials was0.778,the similarity coefficient of wild materials was 0.742,the similarity coefficient of the radiation induced materials from C10 was 0.824,and the similarity coefficient of the radiation induced materials from C2 was 0.791,it means that there was the biggest genetic variation in wild materials and the least genetic variation in radiation induced materials.3）All tested materials were clustered into 6groups by UPGMA dendrogram,the cultivated materials were clustered together,the genetic variation produced by radiation was uncertainty,and the genetic diversity was largely affected by complex geographic landforms and environments of Guizhou plateau.