控制性超排卵和体外成熟培养对卵子线粒体膜电位及细胞骨架的影响

The effect of COH and IVM on mitochondrial membrane potentials and cytoskeleton

目的:探讨不同促卵子成熟方案对卵子线粒体功能的影响和可能机制,为改善当前辅助生殖周期成功率提供新思路。方法:以小鼠为实验动物模型,模拟人类辅助生殖技术(assisted reproductive technology,ART)促卵子成熟的控制性超排卵(COH)和体外成熟培养(IVM)方案,并以自然周期(natural cycle,NC)为对照,采用免疫荧光及化学发光法,检测分析三组卵子线粒体膜电位及卵子细胞骨架。结果:COH组线粒体膜电位显著低于NC组及IVM组(P<0.05);IVM组线粒体膜电位较NC组低,但差异无显著性(P>0.05)。细胞骨架分析显示COH组与IVM组的纺锤体正常率、染色体正常率、纺锤体及染色体均正常率与NC组比较均显著降低(PCOH<0.01,PIVM<0.05)。结论 :COH过程及IVM均会对卵子线粒体功能产生影响。

Objective To investigate the effect of different maturation methods on mitochondrial functions of oocytes and the possible mechanism. To explore novel ideas for developing assisted reproductive technology（ART）. Methods Female mice were used as models and randomly allocated into three groups, COH, IVM and NC control. Oocytes maturated with different methods which were all simulated with those treatments in human IVF cycle. Immunofluorescence were used to measure the mitochondrial membrane potentials and analyze the cytoskeleton. Results The mitochondrial membrane potential in the COH group was significantly lower than that in NC group and IVM group（P 0.05）. The proportion of normal cytoskeleton including spindle structure and chromosome configuration in the COH group and IVM group were significantly lower than that in the NC group（PCOH 0.01, PIVM 0.05）. Conclusions Both COH and IVM can affect mitochondrial functions.