摘要
目的：探讨Src羧基端激酶（C－terminal Src kinase，Src激酶）抑制剂PP2对大鼠C6胶质瘤细胞生物学行为的影响及其机制。方法用浓度为0（溶剂对照组）、2．5、5、7．5 mol／L PP2作用于C6胶质瘤细胞12 h后，分别采用CCK8、划痕实验、transwell基质胶侵袭实验观察PP2对C6胶质瘤细胞形态、增殖、侵袭和迁移的影响。免疫印迹法检测细胞核内β－catenin蛋白表达。结果不同浓度的PP2作用于C6胶质瘤细胞12 h后均能诱导细胞聚集并产生明显的形态改变，且浓度越高，聚集效果越明显。 PP2作用于C6胶质瘤细胞12 h后可明显抑制其增殖、迁移和侵袭，且呈浓度相关性，同时，细胞核内p－β－catenin蛋白表达明显减少。结论 Src激酶抑制剂PP2可能通过减少细胞核内p－β－catenin蛋白表达，从而有效抑制C6胶质瘤细胞的增殖、侵袭和迁移。
Objective To investigate the effects of C -terminal Src kinase inhibitor PP 2 on the biological behavior of rat C6 glioma cells and related mechanism .Methods C6 glioma cells were treated with 0, 2.5, 5, 7.5 mmol/L of PP2 for 12 h.Then, the effects of PPS on the morphological changes , proliferation, invasion and migration of C6 glioma cells were detected by CCK8 assay, wound healing assay and Transwell invasion assay .Furthermore, the level of β-catenin was determined by Western blotting .Results After exposure to various concentrations of PP 2 for 12 h, C6 glio-ma cells showed cell aggregation and remarkably morphological changes , which were more obvious as the concentration of PP2 increased.PP2 also significantly inhibited the proliferation , migration and invasion of C6 glioma cells in a concentra-tion-dependent manner .Meanwhile, the level of β-catenin in the nucleus was significantly decreased .Conclusion PP2 can inhibit the proliferation , migration and invasion of C 6 glioma cells through down -regulation of β-catenin ex-pression in the nucleus .
出处
《徐州医学院学报》
CAS
2016年第7期421-424,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金（81372698）
江苏省肿瘤生物治疗重点实验室课题（ZL1207）
徐州医科大学研究生科技创新计划（xyyc14-11）
