沙棘总黄酮对东莨菪碱所致记忆障碍小鼠的作用及机制

Effects of total flavones of hippophae on scopolamine-induced memory impairment in mice and related mechanism

目的：研究沙棘总黄酮（ total flavones of hippophae ）对东莨菪碱（ scopolamine ）所致记忆障碍小鼠的作用及其与海马内乙酰胆碱酯酶（AChE）浓度的关系。方法将昆明种小鼠随机分为4组（n＝10）：生理盐水（NS）组、东莨菪碱2 mg· kg ^－1（Scop）组、沙棘总黄酮100 mg· kg^－1（Tfh100）组、沙棘总黄酮200 mg· kg^－1（Tfh200）组。在避暗实验中，小鼠分别腹腔注射东莨菪碱建立记忆障碍模型，观察沙棘总黄酮对记忆障碍小鼠潜伏期和错误次数的影响。避暗实验结束后立即断头取脑，分别检测每组小鼠海马中AChE的浓度。结果与NS组相比，Scop组的错误次数明显增多，潜伏期明显缩短，AChE的浓度显著升高（P均＜0．01）；与Scop组相比，Tfh100、Tfh200组潜伏期明显延长，错误次数明显减少，AChE的浓度显著下降（P均＜0．01）。结论沙棘总黄酮对东莨菪碱所致小鼠记忆障碍有保护作用，其机制可能与其降低海马内AChE的浓度有关。

Objective To explore the effects of total flavones of hippophae on scopolamine induced memory impair -ment in mice and the its relationship with the concentration of acetylcholinesterase （ AChE） in the hippocampus .Meth-ods Mice were randomly divided into four groups （n =10）： a normal saline （NS） group, a 2 mg/kg scopolamine （Scop） group, a 100 mg/kg total flavones of hippophae （Tfh100） group and a 200 mg/kg total flavones of hippophae （Tfh200） group.A mouse model of memory impairment was established through intraperitoneal injection of scopolamine . Then, the effects of total flavones of hippophae on the latency and number of errors were observed in the step -through test.Immediately after the step-through test, the brains were removed to detect the concentration of AChE in the hippo-campus of mice .Results Compared with the NS group , the Scop group presented remarkably higher number of errors , shortened latency, and increased concentrations of AChE （P〈0.01）.Compared with the Scop group, the Tfh100 and Tfh200 groups showed markedly extended latency , less number of errors , and decreased concentrations of AChE （ P〈0.01）.Conclusion Total flavones of hippophae have protective effects on scopolamine -induced memory impairment in mice, which may be related to decreased concentration of AChE in the hippocampus .