负载虾青素淀粉纳米粒的表征与稳定性能研究

Characterization and stability of the starch nanoparticles loaded with astaxanthin

为了提高虾青素的稳定性,制备了淀粉接枝聚甲基丙烯酸甲酯纳米粒,然后采用透析法,将虾青素与纳米粒混合制成虾青素纳米粒。测定了淀粉及接枝共聚物的红外光谱、纳米粒的平均粒径、粒径分布、纳米粒形态,并以虾青素的丙酮溶液作为比较,对虾青素纳米粒的稳定性进行了测定。结果表明,虾青素纳米粒的平均粒径为(208.1±26.4)nm,分散系数为0.284±0.035;4 h光照后,虾青素纳米粒保留率为26.34%;30℃和50℃恒温避光下,贮存8 d时虾青素纳米粒的保留率分别为91.81%和83.44%;在p H=1、3、5、7、9或11条件下,虾青素纳米粒的保留率平均值为86.18%。

In order to improve the stability of astaxanthin,the starch- graft- poly（ methyl methacrylate） nanoparticles were synthesized,and then nanoparticles loaded with astaxanthin were prepared by the dialysis method.IR spectra of the starch and the graft copolymer,the average particle size,particle size distribution,and morphology of the nanoparticles loaded with astaxanthin were measured. The stability of nanoparticles loaded with astaxanthin was determined with an acetone solution of astaxanthin as a comparison.The results showed that the average particle size and polydispersity indexof staxanthin nanoparticles were（ 208.1 ± 26.4） nm and 0.284 ± 0.035 respectively.The astaxanthin retention rate of nanoparticles was 26.34% after 4 h under artificial lighting.It was 91.81% and 83.44%after storage 8 days at 30 ℃ and 50 ℃ respectively,in the dark under same temperature conditions.It was 86.18%on average at p H1,3,5,7,9 or 11 conditions.