酸浆五种部位醇提物的活性比较研究

Comparison study on activities of the ethanol extract from five parts of Physali alkekengi L.

目的:探讨酸浆根、茎、叶、宿萼和浆果醇提物的抑菌、抗氧化、抗肿瘤和抑炎活性。方法:采用牛津杯法检测酸浆五种部位醇提物对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的抑菌活性;DPPH清除法检测其抗氧化活性;Alamar Blue法检测其对人乳腺癌细胞株MCF-7、MDA-MB-231和肝癌细胞株Bel7402的抑制效果;一氧化氮检测试剂盒测定其抗炎活性。结果:浆果对金黄色葡萄球菌和大肠杆菌的抑菌效果最好,叶对枯草芽胞杆菌抑菌活性较明显,抑菌圈分别为(16.63±0.49)、(14.97±0.37)、(15.83±0.29)mm。宿萼的抗氧化活性最高,浓度为5 mg/m L时对DPPH的清除率为96.79%±2.09%,基本和同剂量的VC效果相当。酸浆各部位醇提物对肿瘤细胞的抑制作用呈现浓度依赖性,其中根对MCF-7的抑制活性较强,宿萼对MDA-MB-231和Bel7402的活性较强,IC50分别为(31.46±1.49)、(48.84±1.69)、(65.48±1.82)μg/m L。叶的抗炎效果最佳,茎抗炎效果较弱,10μg/m L时NO产生量分别为11.01、27.73μmol/L。结论:酸浆具有一定的抑菌、抗氧化、抗肿瘤和抗炎活性,为酸浆的功能开发及活性成分分离提供一定的理论依据。

Objective： The antimicrobial,antioxidant,anticancer and anti- inflammatory activities of the different parts of Physali alkekengi L. were evaluated in this study. Methods： The antimicrobial activities against Staphylococcus aureus,Escherichia coli and Bacillus subtilis of the alcohol extracts of different parts were investigated using Oxford cup method. The antioxidant activities were evaluated by 2,2- diphenyl- 1- picrylhydrazyl（ DPPH）scavenging assay. The inhibition effects of human cancer cell lines MCF- 7,MDA- MB- 231 and Bel7402 were detected by Alamar Blue assay. NO assay kit was used to determine the anti- inflammatory activity. Results： The alcohol extract of fruit showed the highest antimicrobial activity against S. aureus and E. coli. Besides,the alcohol extract of leaves showed the highest antimicrobial activity against B. subtilis. The diameters of antibacterial circle were（ 16.63 ± 0.49）,（ 14.97 ± 0.37）,（ 15.83 ± 0.29） mm,respectively.The alcohol extract of calyx exhibited 96.79% ±2.09% DPPH radical cavenging efficiency at the concentration of 5 mg / m L,which demonstrated the similar antioxidant activity with the same dose of VC.All alcohol extracts of five parts showed inhibition activities on human cancer cells in a dose- dependent manner.The alcohol extracts of leaves showed better inhibition activities against MCF-7.Furthermore,the calyx showed better inhibition activities against MDA- MB-231 and Bel7402.The IC50were（ 31.46 ± 1.49）,（ 48.84 ± 1.69） and（ 65.48 ± 1.82） μg /m L.In addition,the alcohol extract of leaves showed the highest anti-inflammatory activity and the stem was lowest.NO production were 11.01 and 27.73 μmol / L at the concentration of 10 μg / m L.Conclusion： P.alkekengi L.has antibacterial,antioxidant,anti- tumor and anti- inflammatory activity.These results provided theoretical basis for the functional development,further utilization of P.alkekengi L.