子痫前期与COMT基因Val158Met多态性的相关性研究

ASSOCIATION STUDY BETWEEN COMT GENE VAL158MET POLYMORPHISM AND PREECLAMPSIA

目的:研究儿茶酚胺氧位甲基转移酶(COMT)基因rs4680位点Val158Met(G→A转换)多态性与子痫前期的关联。方法:应用聚合酶链式反应—限制性片段长度多态性(PCR—RFLP)技术,检测80例正常妊娠妇女(对照组)及70例子痫前期患者(病例组)的COMT基因Val158Met多态位点基因型及等位基因分布频率。结果:COMT基因Val158Met多态位点G、A等位基因在两组的分布频率比较,差异无统计学意义(P>0.05)。COMT GG、GA、AA基因型分布频率,两组比较差异亦无统计学意义(P>0.05)。结论:COMT基因Val158Met(G→A转换)多态位点可能与子痫前期的发病无关。

Objective To analyze the association of catechol-O-methyltransferase （COMT） gene Val158M etpolymorphism （rs4680 G 〉 A ） and preeclampsia. Methods： COMT gene Val158M et polymorphism was detectedby polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 70 cases ofpregnant patients with preeclampsia and 80 healthy pregnant women. Results ： There was no significantdifference in the prevalence of G /A allele of the COMT gene between preeclampsia patients and the controls（ P 〉 0 . 05）. There was no statistical difference in the prevalence of GG, GA and A A genotypes of COMTVal158M et polymorphism between the two groups （ P 〉 0 . 05）. Conclusion： COMT gene Vail 58Met polymorphism（ O A ） may have no association with preeclampsia.