延龄草对LPS诱导大鼠肝损伤的保护作用及机制

The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide

目的：研究延龄草（TIM）对脂多糖（LPS）诱导大鼠氧化应激与肝损伤的保护作用。方法：SD大鼠60只，按体重随机分成刑高、中、低剂量组、模型组、地塞米松磷酸钠（DEX）对照组及空白对照组（n=10）。TTM高、中及低剂量组按（8、4、2）g/（kg·d）TTM灌胃，模型组、DEX对照组及空白对照组灌胃等量蒸馏水，每隔5d，TTM高、中、低剂量组、模型组、DEX对照组按1mg／kg腹腔注射LPS，DEX对照组同时腹腔注射DEX（5mg／kg），空白对照组注射等量生理盐水。30d后，测定大鼠胸腺指数、脾脏指数，对血清一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）活性与一氧化氮（NO）、谷胱甘肽（GSH）、硫代巴比妥酸反应产物（TBARS）、白细胞介素6（IL-6）、IL-10及肿瘤坏死因子α（TNF-α）含量，肝组织SOD、谷胱甘肽过氧化氢酶（GSH—Px）活性与GSH、TBARS含量进行检测。结果：与模型组相比，TTM高剂量组在（19～30）d体重显著降低（P〈0．05），TTM高、中、低剂量组胸腺指数，TTM高剂量组脾脏指数显著降低（P〈0．05），TTM高、中、低剂量组血清NOS活性与TBARS、NO含量显著降低（P〈0．05），TTM高剂量组血清SOD活性及中、高剂量组GSH含量显著上升（P〈0．05），TTM高、中剂量组血清IL-6、TNF-α含量显著降低，IL-10含量显著升高（P〈0．05），TTM中、高剂量组肝脏TBARS含量显著降低，硎各剂量组肝脏SOD活性与中、高剂量组GSH—Px活性，高剂量组GSH含量显著升高（P〈0．05）。结论：TTM对LPS所致大鼠的胸腺、脾脏萎缩有一定的延缓作用，能有效降低血清中NOS活性，减少NO生成，提升SOD、GSH—Px活性与GSH含量，减轻脂质过氧化，降低IL-6、TNF-α过量分泌、提升IL-10含量．有抗炎护肝的功能．

Objective： To study the protective effects of Trillium tschonoskii maxim （TIM）on rats＇ oxidative stress and hepatotoxicity induced by lipopolysaceharide （LPS）. Methods： Sixty SD rats were randomly divided into TIM high, medium and low close groups, model group, Dexamethasone （DEX） control group and blank control group with ten rats in each group. The TIM high, medium and low dose groups were treated with 8, 4, 2 g/（ kg·d）TTM by intragastric administration and model group, DEX control group and blank control group were treated with the same amount of distilled water respectively. The TIM high, medium and low dose groups, model group, DEX control group were injected intraperitoneal with 1 mg/kg LPS and the DEX control group was injected intraperitoneal with 5 mg/kg DEX, the blank control group was injected with same amount of normal saline every five days. The indexes of rats＇ thymus and spleen were measured in 30 days. The activities of serum nitric oxide synthase （NOS）, superoxide dismutase （SOD） and the contents of nitric oxide （NO）, glutathione （GSH）, glueosinolates barbiturie acid reaction produet（TBARS）, white cells interleukin-6（IL-6）, IL-10 and tumor necrosis factor-α（TNF-α）, the activi- ties of liver SOD, GSH-Px and the contents of GSH and TBARS were measured. Results： TIM high dose group was significantly different in body weight in 19 - 30 days（ P 〈 0.05） ; The index of thymus in TTM high, medium and low close groups and the index of spleen in TIM high dose group were decreased significantly compared with those of the model group. The activity of serum NOS and the contents of TBARS and NO in TIM high, medium and low dose groups were decreased significantly（ P 〈 0.05）. The activity of serum SOD in TFM high dose group and the contents of GSH in TIM medium and low dose groups were increased significantly（ P 〈 0.05）. The contents of serum IL-6 and TNF-α in TIM high, medium dose groups were decreased significantly and the contents of serum IL-10 were increased significantly（ P 〈 0.05）. The contents of liver TBARS in TTM high, medium dose groups were decreased significantly. The activity of liver SOD in TIM high, medium and low dose groups, the activity of GSH-Px in TTM high, medium dose groups and the contents of GSH in TTM high dose group were increased significandy（ P 〈 0.05）. Conclusion： TFM has a certain effect to delay the rats＇ atrophy of thymus and spleen generated by LPS. It can effectively reduce the activity of NOS in serum, reduce the formation of NO, improve the aetivity of SOD, GSH-Px and the contents of GSH, reduce lipid peroxidation, decrease the excessive secretion of IL-6, TNF-α, increase the contents of IL-10, which can resist inflammation and protect the liver.