Ras蛋白特异性因子RASGRF1在结直肠癌组织中的表达及影响因素

Clinical study of expression levels of RASGRF1 in colorectal cancer tissues

目的探讨Ras蛋白特异性的鸟苷酸释放因子1(RASGRF1)在结直肠癌组织中的表达水平及低表达的相关影响因素。方法选择2012年4月至2015年1月收治的62例结直肠癌患者为研究对象,均通过外科手术获得结直肠癌组织及癌变病灶旁正常结肠组织样本,免疫组织化学法检测组织样本中RASGRF1表达水平。根据检查结果将受试者分成低表达组(阴性或+)和高表达组(++或+++)两组,回顾性分析其临床资料,对单因素分析后存在显著性差异的指标行非条件Logistic回归分析。结果 1结直肠癌组织中RASGRF1阳性表达率为59.7%(37/62),显著低于癌旁正常结肠组织的96.8%(60/62)(c2=25.046,P=0.000);结直肠癌组织RASGRF1低表达率为61.3%(38/62),明显高于癌旁正常结肠组织的41.9%(26/62)(c2=4.650,P=0.031);2低表达组患者38例中女24例(63.2%),TNM分期为Ⅲ-Ⅳ期者28例(73.7%),远处转移者25例(65.8%),均显著高于高表达组24例患者中的11例(45.8%)、6例(25.0%)和4例(16.7%),差异具有统计学意义(P<0.05);3性别(女)、TNM分期(Ⅲ-Ⅳ期)及远处转移是结直肠癌组织中RASGRF1低表达的相关影响因素(P<0.05)。结论结直肠癌患者癌组织中RASGRF1表达水平较低,TNM分期、远处转移、性别均为RASGRF1低表达的相关影响因素。

Objective To investigate the expression levels of Ras guanine nucleotide release factor 1 （RASGRF1） in colorectal cancer tissues and the related influencing factors of its low expression. Methods Sixty-two cases with colorectal cancer were selected as the research objects from April 2012 to January 2015. The colorectal cancer tissues and normal colon tissue samples beside cancerous lesions were obtained by surgery. The expression levels of RASGRF1 in tissue samples were detected by immunohistochemistry. According to the results of examination, the subjects were divided into the low expression group （group A） and high expression group （group B）. The clinical data were retrospectively analyzed. For those indexes with significant differences after univariate analysis, non-conditional Logistic regression analysis was performed. Results ①The positive expression rate of RASGRF1 in colorectal cancer tissues （59.7%） was significantly lower than that in normal colon tissues beside cancerous lesions （96.8%） （χ^2=25.046, P=0.000）. The low expression rate of RASGRF1 in colorectal cancer tissues （61.3%） was significantly higher than that in normal colon tissues beside cancerous lesions （41.9%） （χ^2=4.650, P=0.031）.②In group A （38 cases）, there were 24 female cases （63.2%）, 28 patients （73.7%） at stageⅢ-Ⅳof＆amp;nbsp;TNM staging and 25 patients （65.8%） with distant metastases, which were all significantly higher than those in group B [24 cases totally, 11 cases （45.8%）, 6 cases （25.0%）, and 4 cases （16.7%）, respectively] （P〈0.05）.③Non-conditional Logistic regression analysis showed that gender （female）, TNM staging （stageⅢ-Ⅳ） and distant metastasis were the related influencing factors of low expression of RASGRF1 in colorectal cancer tissues （P〈0.05）. Conclusions The expression level of RASGRF1 in colorectal cancer tissues is lower. TNM staging, distant metastasis and gender are the related influencing factors of low expression of RASGRF1 in colorectal cancer tissues.