仙茅多糖的分离纯化及结构分析

Isolation,Purification and Structural Analysis of Curculiginis Rhizoma Polysaccharide

目的:对仙茅多糖进行分离纯化,并对其结构进行分析,为仙茅多糖的进一步应用提供理论基础。方法:仙茅根茎经水提、醇沉、冷冻干燥得仙茅粗多糖(XMP),经DEAE-纤维素柱和Sepharose CL-6B凝胶柱色谱纯化得纯多糖XMP-1,采用凝胶渗透色谱、紫外光谱、红外光谱、气质联用等技术对XMP-1的结构进行分析。结果:仙茅多糖由甘露糖、葡萄糖、半乳糖3种单糖组成,其摩尔比为81.2∶5.7∶1,多糖质量分数为91.8%,相对分子质量为3 031 Da,不含核酸、蛋白质和色素。具有吡喃特征吸收峰,在水溶液分子中的分子构象可能为自然卷曲结构。结论:仙茅纯多糖是一种分子量较小的中性杂多糖。

Objective： To study the isolation, puriffication and structural analysis of the polysaccharides from the Curculiginis Rhizoma. The work will provide the theoretical foundation for the further application of Curculiginis Rhizoma polysaccharides. Method： The crude polysaccharide was obtained by hot water extraction, ethanol precipitation and freezedrying. After further purification by DEAE-cellulose and Sepharose CL-6B column chromatography, pure polysaccharide （XMP-1） was achieved. The structural of XMP-1 was elucidated by GPC, UV, IR and GC-MS. Result： XMP-1 was composed of mannose, glucose, galactose with a molar ratio of 81.2：5.7：1. Total carbohydrate content of XMP-1 was 91.8% and the molecular weight was 3 031 Da. XMP-1 have no nucleic aicd, protein and pigment. IR chromatography showed that XMP-1 has pyrane characteristic absorption peak. XMP-1 in molecular conformation in aqueous solution can be naturally curly structure. Conclusion： XMP-1 is a kind of small molecular weight neutral pure heterosaccharides.