期刊文献+

垂体肿瘤转化基因1在雄激素非依赖性前列腺癌发生过程中的表达变化 被引量:4

Expression of pituitary tumor-transforming gene 1 during the development of androgen-independent prostate cancer
下载PDF
导出
摘要 目的:探讨垂体肿瘤转化基因1(PTTG1)在前列腺癌由激素依赖性转化为激素非依赖性过程中的表达变化。方法:通过在去雄激素培养条件下长期培养雄激素依赖性前列腺癌细胞株LNCa P,建立并验证雄激素非依赖性亚系LNCa P-AI细胞模型,用Western印迹、RT-PCR方法每2周检测在去雄激素培养过程中PTTG1的表达改变。结果:经过3个月培养,成功建立雄激素非依赖性亚系LNCa P-AI细胞模型;在LNCa P细胞中,随着去雄激素培养时间延长,PTTG1的表达水平逐渐升高,基质金属蛋白酶-2(MMP-2)和MMP-9表达有同步升高趋势。结论:PTTG1表达在雄激素非依赖性前列腺癌发生过程中逐渐增强,可能是晚期前列腺癌基因治疗的靶标之一。 Objective: To explore the expression of pituitary tumor transforming gene 1(PTTG1) during the transformation of prostate cancer from androgen-dependent( ADPC) to androgen-independent( AIPC). Methods: We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.Results: The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and-9 were elevated at the same time. Conclusion: The expression of PTTG1 is increased gradually in AIPC,which may be a target of gene therapy for advanced prostate cancer.
出处 《中华男科学杂志》 CAS CSCD 北大核心 2016年第8期686-691,共6页 National Journal of Andrology
基金 南京军区医学科技创新课题(11MB006)~~
关键词 前列腺癌 雄激素非依赖性 垂体肿瘤转化基因1 雄激素剥夺治疗 LNCAP细胞 基质金属蛋白酶-2 基质金属蛋白酶-9 prostate cancer androgen-independent pituitary tumor transforming gene 1 androgen deprivation therapy LNCa P cell line matrix metalloproteinases 2 matrix metalloproteinases 9
  • 相关文献

参考文献22

  • 1Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015.CA Cancer J Clin,2016,66(2):115-132.
  • 2马春光,叶定伟,李长岭,周芳坚,姚旭东,张世林,戴波,张海梁,朱耀,沈益君.前列腺癌的流行病学特征及晚期一线内分泌治疗分析[J].中华外科杂志,2008,46(12):921-925. 被引量:123
  • 3叶定伟,朱耀.中国前列腺癌的流行病学概述和启示[J].中华外科杂志,2015,0(4):249-252. 被引量:268
  • 4Tong Y,Eigler T.Transcripttional targets for pituitary tumortransforming gene-1.J Mol Endocrinol,2009,43(5):179-185.
  • 5Lee SO,Dutt SS,Nadiminty N,et al.Development of an androgen-deprivation induced and androgen suppressed human prostate cancer cell line.Prostate,2007,67(12):1293-1300.
  • 6Detchokul S,Elangovan A,Crampin EJ,et al.Network analysis of an in vitro model of androgen-resistance in prostate cancer.BMC Cancer,2015,15:883.
  • 7Burton DG,Giribaldi MG,Munoz A,et al.Androgen deprivation-induced senescence promotes outgrowth of androgen-refractory prostate cancer cells.PLo S One,2013,8(6):e68003.
  • 8Xiao JQ,Liu XH,Hou B,et al.Correlations of pituitary tumor transforming gene expression with human pituitary adenomas:A meta-analysis.PLo S One.2014,9(3):e90396.
  • 9Liang M,Chen X,Liu W,et al.Role of the pituitary tumor transforming gene 1 in the progression of hepatocellular carcinoma.Cancer Biol Ther,2011,11(3):337-345.
  • 10Wang X,Duan W,Li X,et al.PTTG regulates the metabolic switch of ovarian cancer cells via the c-myc pathway.Oncotarget,2015,6(38):40959-40969.

二级参考文献36

  • 1邵常霞,项永兵,刘振伟,高静,孙璐,方茹蓉,阮志贤,高立峰,金凡,高玉堂.上海市区泌尿系统恶性肿瘤相对生存率分析[J].中国肿瘤临床,2005,32(6):321-324. 被引量:31
  • 2王晓雄,黄亮,洪宝发,文载律,刘悦,朱开常.前列腺癌根治术后无瘤生存期的影响因素分析[J].中华泌尿外科杂志,2005,26(6):379-382. 被引量:3
  • 3叶定伟.前列腺癌的流行病学和中国的发病趋势[J].中华外科杂志,2006,44(6):362-364. 被引量:168
  • 4高江平,洪宝发.前列腺癌内分泌治疗的进展[J].中华外科杂志,2006,44(6):393-395. 被引量:7
  • 5Parkin DM, Bray F, Ferlay J, et al. Global cancer statistics, 2002. CA Cancer J Clin, 2005, 55:74-108.
  • 6Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2006. CA Cancer J Clin, 2006, 56:106-130.
  • 7Michael PM, Bernard D, Kaill M, et al. Management of prostate cancer in China: a multicenter report of 6 institutions. J Urol, 2005, 174:1794-1797.
  • 8Murphy AM, McKiernan JM, Olsson CA. Controversies in prostate cancer screening. J Urol, 2004, 172 : 1822-1827.
  • 9Benaim EA, Pace CM, Roehrbor CG. Gleason score predicts androgen independent progression after androgen deprivation therapy. Eur Urel, 2002, 42:12-17.
  • 10Benaim EA, Pace CM, Lam PM, et al. Nadir PSA as a progression to androgeindependent prostate cancer. Urology, 2002, 59:73-77.

共引文献376

同被引文献44

引证文献4

二级引证文献16

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部