摘要
目的:探讨垂体肿瘤转化基因1(PTTG1)在前列腺癌由激素依赖性转化为激素非依赖性过程中的表达变化。方法:通过在去雄激素培养条件下长期培养雄激素依赖性前列腺癌细胞株LNCa P,建立并验证雄激素非依赖性亚系LNCa P-AI细胞模型,用Western印迹、RT-PCR方法每2周检测在去雄激素培养过程中PTTG1的表达改变。结果:经过3个月培养,成功建立雄激素非依赖性亚系LNCa P-AI细胞模型;在LNCa P细胞中,随着去雄激素培养时间延长,PTTG1的表达水平逐渐升高,基质金属蛋白酶-2(MMP-2)和MMP-9表达有同步升高趋势。结论:PTTG1表达在雄激素非依赖性前列腺癌发生过程中逐渐增强,可能是晚期前列腺癌基因治疗的靶标之一。
Objective: To explore the expression of pituitary tumor transforming gene 1(PTTG1) during the transformation of prostate cancer from androgen-dependent( ADPC) to androgen-independent( AIPC). Methods: We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.Results: The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and-9 were elevated at the same time. Conclusion: The expression of PTTG1 is increased gradually in AIPC,which may be a target of gene therapy for advanced prostate cancer.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2016年第8期686-691,共6页
National Journal of Andrology
基金
南京军区医学科技创新课题(11MB006)~~