超高效液相色谱检测乳粉复原乳中的氨基酸

Determination of amino acids in spray-dried milk by UPLC

建立超高效液相色谱法（UPLC）测定乳制品中多种氨基酸,并对不同温度喷雾干燥的乳粉再制复原乳中氨基酸含量进行检测,以期通过乳中氨基酸含量和组成的变化鉴别复原乳。结果表明：17种氨基酸在0.003 7~0.052 2 mg/m L内有良好的线性关系,回收率为97.45%~105.65%。该检测方法灵敏度高,准确性好;不同温度喷雾干燥乳粉中各氨基酸含量不同,其中谷氨酸、亮氨酸、半胱氨酸的变化最为明显。该检测可作为复原乳的鉴别方法。

Established an UPLC method in determination the amino acids of dairy products,and detected the amino acid content of dairy products processed in different temperature,to identify the spray dried milk through the changes of amino acids contents and composition. The results showed that the calibration curve was linear in the range of 0. 003 7 ~ 0. 052 2mg / m L of amino acids,the average recovery range of amino acid was from97. 45% ~ 105. 65%. It has the advantage of simple,rapid and accuracy in the determination amino acids. With the spray temperature changing,the content of amino acids were different. And the change of Glu,Leu,Cys were the most obvious. It can serve as the distinguish method of reconstituted milk.