丁香油微乳的制备及抑菌活性研究

Study on the preparation and antibacterial activity of clove oil microemulsions

为了改善丁香油水相溶解性及储藏稳定性差的应用缺陷,以可稀释微乳包载丁香油。通过比较丁香油微乳拟三元相图中微乳区面积(S_(ME))和最小可稀释比(DR),选择聚氧乙烯蓖麻油(Cremophor)EL 30与无水乙醇以21(质量比)为混合表面活性剂,水相环境为中性制备丁香油微乳。进一步采用电导率法和流变法对微乳构型进行鉴定,并选择O/W中心区域微乳:混合表面活性剂/丁香油/水(质量比)=16/4/80,Km=2,进行抑菌活性评价。结果表明,微乳包埋体系并未改变丁香油的抑菌活性。同时,通过检测丁香油微乳在稀释和储藏过程中的粒径变化,发现此微乳能保持良好的稀释和储藏稳定性。因此,该微乳在有效改善丁香油的水溶性和储藏稳定性的同时,对其抑菌活性没有影响。

In order to improve the stability and water solubility of clove oil,fully dilutable microemulsions incorporating clove oil were prepared in this study. By comparing the areas of microemulsion region in pseudo-ternary phase diagram（ S_（ME）） and the minimum dilution ratio（ DR）,Cremophor EL 30 and anhydrous ethanol were chosen to prepare clove oil microemulsion at 2： 1（ w： w）,and the aqueous phase was neutral. Further,the type of microemulsion were identified using electric conductivity and rheometer,and the antibacterial activity of microemulsion in O / W central zone was also determined,with mixed surfactant / clove oil / water at 16 /4 /80（ w / w / w）,Km = 2. The results revealed that the encapsulation showed little effenct on the antibacterial activity of clove oil. Meanwhile,no significant changes of microemulsion size were determined when diluting and storing,and this might indicate that microemulsion maintained a fine dilution and storage stability. Thus,clove oil microemulsions could improve water solubility and storage stability of clove oil with no effect on their antimicrobial activities.