层层叠加制备三维静电纺纳米纤维结构

Fabrication of 3D Electrospun Nanofiber Structures Through Stacking Multi-Layers Fiber Membrane

以通孔金属片为静电纺丝的负极制备了通孔阵列纳米纤维薄膜,将多层纳米纤维薄膜在溶剂中叠加构建了三维纳米纤维结构。扫描电子显微镜结果表明,对于聚苯乙烯、聚乙烯吡咯烷酮和聚己内酯3种不同的高分子材料,均可形成带有规则有序通孔结构的纳米纤维薄膜,孔的大小可以通过模板的选择进行调节。在水中将聚苯乙烯纤维薄膜层层叠加形成了三维纳米纤维结构。在叠加四层聚苯乙烯纤维薄膜的三维结构上培养NIH3T3细胞,细胞可以在三维空间内生长,三维纤维结构表现出良好的生物相容性。

Nanofiber mats with through-hole patterns were prepared by electrospinning technique, in which a metal plate with hole arrays was used as negative collector. Afterwards, 3D nanofiber structures were fabricated by stacking different layers nanofiber mats in solvent. Scanning electron microscopy measurement states that nanofiber membrane with regular holes is formed for different polymers, such as polystyrene （PS）, polyvinyl pyrrolidone （PVP） and polycaprolactone （PCL）. The size of the hole in the nanofiber film can be controlled by the original design of the negative collector. Obvious 3D structure was constructed after stacking four nanofibrous layers polystyrene fiber mats together in water. The PS 3D nanofiber structures show high biocompatibility for the NIH3T3 cell culture, which is able to grow towards different directions.