化妆品中动物源性成分多重实时荧光PCR检测方法的研究

Study of multiplex real-time fluorescent PCR detection method for animal originated components in cosmetics

使用优化的Chelex-100结合玻璃奶法提取膏状、液态和固态化妆品中的动物源性DNA;根据驴、马和牛线粒体16S rRNA基因序列设计通用引物和特异性探针,建立了一次性检测化妆品中驴、马和牛3种动物源性成分的多重实时荧光PCR体系,检出限均为0.001 ng。并对市售的面霜、洗面奶和面膜进行盲样检测,验证了体系的可靠性和准确性。

DNA of animal originated components in paste,liquid and solid form cosmetic products were sampled using improved Chelex - 100 combined with Glass Milk method. According to the donkey, horse and cattle mitochondrial 16S rRNA gene sequences, the universal primers and specific probes were designed. Then a multiplex real -time fluorescent PCR assay system to detect the species of animal originated （donkey, horse and cattle） components in cosmetic products was established. All the detection limit of all cosmetics achieves 0. 001 rig. Samples from maTketed cosmetics such as creams,lotions and facial masks were detected by this way. The reliability and accuracy of this multiplex real - time fluorescent PCR assay were confirmed.