刺玫果总黄酮的双水相萃取工艺及其抗氧化能力

Aqueous Two-phase Extraction of Total Flavonoids in Fruit of Rosa davurica Pall. and Its Antioxidant Ability

为探索刺玫果总黄酮的乙醇-硫酸铵双水相(添加氯化钠)萃取工艺及其体外抗氧化性。采用单因素和正交试验法对刺玫果总黄酮的双水相萃取工艺进行了优化;并通过测试刺玫果总黄酮提取液对二苯基苦基肼自由基(DPPH·)、羟基自由基(·OH)、超氧阴离子自由基(O-2·)、亚硝酸盐、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵自由基阳离子(·ABTS+)的清除率和还原力,评价其抗氧化活性。结果表明:在乙醇体积分数27%、硫酸铵质量浓度0.21 g/m L、刺玫果提取液体积分数22%、氯化钠加入量为2.0 g的最佳工艺条件下刺玫果总黄酮平均萃取率为91.81%。刺玫果总黄酮提取液对·ABTS+、·OH和O-2·具有显著的清除能力,当总黄酮质量浓度分别为0.12、0.30和0.60 g/L时,对·ABTS+、·OH和O-2·的清除率分别为90.27、89.60%和96.73%,其清除能力均强于Vc;对DPPH·、亚硝酸盐具有一定的清除能力,但弱于Vc;刺玫果总黄酮提取液具有一定的还原能力。

In order to study the aqueous two-phase extraction of total flavonoids in fruit of Rosa davurica Pall. , both single factor and orthogonal test methods were applied to optimize the extraction conditions. The antioxidant ability of total flavonoids was evaluated according to the clearance ratio and reducing power to superoxide anion free radicals, DPPH free radical, hydroxyl free radical, sodium nitrite and ABTS radical cations. The results showed that the optimum extraction conditions were the volume fraction of absolute ethyl alcohol 27%, mass concentration of ammonium sulfate 0.21 g/mL, volume fraction of extraction solution 22%, and addition of sodium hydroxide 2.0 g, respectively. The average extraction ratio could reach 91.81% under the optimum extraction conditions. The extraction solution showed significant clearance ability to ABTS radical cations, hydroxyl free radical, superoxide anion free radicals with the clearance rates of 90.27%, 89.60% and 96.73% as the mass concentrations of total flavonoids 0.12, 0.30 and 0.60 g/L, respectively. They were stronger than those of Vc. The clearance ability of extraction solution to DPPH free radical and nitrite was weaker than that of Vc. The extraction solution also had some reducing power.