T-CAM表面修饰n-HA/PA66复合骨修复材料修复兔桡骨骨缺损的实验研究

The experimental research of T-CAM surface modification nano-hydroxyapatite/polyamide 66 composite materials repair of rabbit radius bone defect

目的探讨T-CAM表面修饰纳米羟基磷灰石/聚酰胺66(nano-hydroxyapatite/polyamide 66,n-HA/PA66)复合骨修复材料修复兔桡骨中段骨缺损的疗效。方法选择36只成年新西兰大白兔,建立右桡骨中段1.5cm骨缺损模型后分为两组:实验组植入T-CAM表面修饰n-HA/PA66复合骨修复材料;对照组植入n-HA/PA66。观察术前,术后2、4、8、12周静脉血碱性磷酸酶(ALP)值;术后4、8、12周每个时间点处死6只兔子,进行X线片、大体标本观察、组织学等观察桡骨骨缺损愈合情况。结果所有兔子术后切口均一期愈合,无死亡,无体内植入材料脱落。影像学及组织学结果表明实验组在术后4周就有新生骨组织,随着时间的延长,实验组新骨生长速度和数量明显优于对照组,术后12周Lane-Sandhu法X线片和组织学评分实验组均高于对照组(P<0.05);术后2周实验组ALP值开始升高,随着时间的推移,两组ALP值均升高,术后8周达最高峰,之后开始下降,在12周时接近于术前,静脉血ALP值在术后第2、4、8周与对照组比较差异有统计学意义(P<0.05)。结论 T-CAM表面修饰n-HA/PA66复合骨修复材料具有促进骨质愈合的作用,尤其在早期修复骨缺损的效果优于n-HA/PA66。

Objective To discuss the efficacy of T-CAM surface modification nano-hydroxyapatite/polyamide 66 （T-CAM/n- HA/PA66） composite materials on the recovery of rabbit radius bone defect. Methods A total of 36 New Zealand rabbits were ar- tificially made to be bone defect by resecting the 1.5 em substantia ossea with periosteum of radius, and were divided into two groups averagely depending on implanted materials：experimental group （T-CAM/n-HA/PA66）, control group （n-HA/PA66）. The alkaline phosphatase （ALP） of venous blood was observed at preoperative and week 2,4,8,12 postoperative. Every 6 rabbits were sacrificed on week 2,4,8,12, and the radius healing status was observed by X-ray, histology. Results No rabbit was infected or died, no implant material was dropped. Gross observation, X-ray results and histology results demonstrated that the experimental group began to have a new bone tissue at 4 weeks after the operation;with the extension of time, the experimental group＇s new bone growth speed and the quantity were better than the those of control group. The Lane -Sandhu method X-ray score showed that there were significant differences between two groups （P〈0. 05） ,as well as the Lane-Sandhu method histological score. The ALP value of experimental group began to rise at two weeks postoperative; with the extension of time, ALP value of both groups increased, peaked at 8 weeks, then＇began to decline,lastly close to the preoperative at 12 weeks, The ALP value at 2,4 and 8 weeks were sig- nificant between two groups （P〈0.01）. Conclusion T-CAM/n-HA/PA66 composite materials could accelerate the healing of bone defect, the effect of repair of bone defect is better than that of n-HA/PA66 artificial bone in the early.