新型纳米银壳聚糖敷料的应用研究

Application research of a novel silver nanoparticles chitosan dressing

目的观察并评价新型纳米银壳聚糖敷料的物理特性、细胞毒性、抑菌性及其对伤口愈合的影响。方法将纳米银材料添加到壳聚糖中制备新型复合生物抗菌敷料,并通过使用电子显微镜、CCK-8、抑菌圈及平板抑菌法、大鼠创伤动物模型愈合试验将壳聚糖敷料、纳米银壳聚糖敷料、银离子敷料及普通纱布敷料进行对比,以评价新型纳米银壳聚糖敷料的物理特性、细胞毒性、抑菌性及对伤口愈合的影响。结果电子显微镜提示纳米银颗粒在壳聚糖敷料表面均匀分散,粒子直径约50nm;抑菌圈及平板抑菌法提示新型纳米银壳聚糖敷料对金黄色葡萄球菌、大肠杆菌及铜绿假单胞杆菌均有较强的抑菌能力;CCK-8实验提示其在0.5%及1.0%的纳米银浓度下无明显细胞毒性;动物愈合实验提示纳米银壳聚糖敷料可以促进伤口愈合。结论纳米银壳聚糖敷料符合现代新型生物敷料的基本要求,相较传统敷料有其优越性。

Objective To evaluate the construction,cytotoxicity,antibacterial activity and wound healing application of a no- vel silver nanoparticles chitosan dressing. Methods Silver nanoparticles were added into chitosan to prepare a new compound bio- logical antimicrobial dressing（silver nanoparticles chitosan dressing,SNC dressing）. By using UV-Vis spectroscopy, scanning elec- tron microscopy, CCK-8 test, zone of inhibition test, quantitative test and wound healing experiment, we evaluated the physical prop- erty of the new dressing and compared this new dressing with traditional dressing,chitosan dressing and silver ion-releasing chit0san dressing. Results UV-Vis Spectroscopy and SEM studies showed silver nanoparticles well dispersed in chitosan fiber with a parti- cle size about 50 nm. In antimicrobial test,the SNC dressing showed a immense antimicrobial property for Staphylococcus aureus, escherichia coli and pseudomonas aeruginosa. In cytotoxicity test, the SNC dressing loading 0.5% and 1.0% silver nanoparticles was proved to behaved slight cytotoxicity. In wound healing experiments, healing promotion and improved reconstruction were ob- served in the SNC dressing compared with other dressings. Conclusion SNC dressing meets the basic requirements of the modern biological dressing and it might have more advantages comparing with traditional dressings.