胰腺癌细胞中TGF-β1对Foxp3表达的影响被引量：2

下载PDF

导出

摘要目的探讨胰腺癌细胞分泌的转化生长因子β1(TGF-β1)对正常人外周血单个核细胞(PBMC)转录因子Foxp3表达的影响。方法利用Transwell小室建立胰腺癌细胞CF-PAC1与PBMC的共培养体系,分为A组(PBMC),B组(PBMC-CFPAC1),C组[PBMC-CF-PAC1+TGF-β1抗体(1μL)],D组[PBMC-CF-PAC1+TGF-β1抗体(2μL)],E组[PBMC-CF-PAC1+TGF-β1抗体(4μL)],F组[PBMC-CF-PAC1+TGF-β1抗体(8μL)],G组[PBMC-CF-PAC1+TGF-β1抗体(16μL)]。采用酶联免疫吸附法检测共培养体系中加入TGF-β1抗体前、后细胞培养液中TGF-β1的水平。采用Western blot法、免疫荧光方法检测共培养体系中加入TGF-β1抗体前、后PBMC中Foxp3的表达量。结果 A组TGF-β1平均浓度为(12.44±2.08)ng/mL,B组TGF-β1平均浓度为(14.12±1.25)ng/mL,两组比较差异有统计学意义(P<0.05)。随着加入TGF-β1抗体浓度的增加,各组TGF-β1水平呈递减趋势(P<0.05)。B组PBMC中Foxp3蛋白相对表达量明显高于A组(P<0.05),其余6组PBMC细胞中Foxp3蛋白的表达量随着加入TGF-β1抗体浓度的增加,呈递减趋势(P<0.05)。结论胰腺癌细胞可通过调整肿瘤微环境中TGF-β1影响PBMC细胞中Foxp3蛋白的表达。

作者曹亚峰衣昕

机构地区同济大学附属杨浦区中心医院血液科南通大学医学院人体解剖学系

出处《重庆医学》 CAS 北大核心 2016年第23期3290-3292,共3页 Chongqing medicine

关键词胰腺肿瘤受体转化生长因子Β 外周血单个核细胞转化生长因子Β1 FOXP3

分类号 R735.9 [医药卫生—肿瘤]

引文网络
相关文献

参考文献8

1Sakaguchi S, Miyara M, Costantino CM, et al. Foxp3+ regulatory T cells in the human immune system[J]. Nat Rev Immunol, 2010,10 ( 7 ) : 490-500.
2Sakaguchi S. Naturally arising Foxp3-expressing CD25+ CD4+ regulatory T cells in immunological tolerance to self and non-self [J]. Nature Immunol, 2005,6 (4) : 345- 352.
3Zhou X,Bailey-Bucktrout S,Jeker LT, et al. Plasticity of CD4 Foxp3 T cells[J]. Cur ()pin Immunol, 2009,21 (3):281.
4Valzasina B, Piconese S, Guiducci C, et aI. Tumor-induced expansion of regulatory T cells by conversion of CD4 CD25 lymphocytes is thymus and proliferation independ- ent[J]. Cancer Res,2006,66(8) :4488-4495.
5Attisano L, Wrana JL. Signal transduction by the TGF-I superfamily [J]. Science,2002,296(5573) :1646-1647.
6Petersen RP,Campa MJ ,Sperlazza J ,et al. Tumor infiltra- ting Foxp3+ regulatory Tcells are associated with recur- rence in pathologic stage ] NSCLC patients [J]. Cancer, 2006,107 (12) : 2866-2872.
7Perrone G, Ruffini PA, Catalano V, et al. Intratumoural Foxp3- positive regulatory T cells are associated with ad- verse prognosis in radically resected gastric cancer [J]. Eur J Cancer,2008,44(13) ..1875k1882.
8Moo-Young TA, I.arson JW, Belt BA, et al. Tumor de- rived TGF-B mediates conversion of CD4 Foxp3+ regu- latory T cells in a murine model of pancreas cancer [J]. J Immunoth, 2009,32( 1 ) : 12-21.