急性白血病患者血清及脑脊液中基质细胞衍生因子-1α的含量及其意义

SDF-1α in Serum and Cerebrospinal Fluid of Acute Leukemia Patients and Its Significance

目的检测急性白血病患者血清及脑脊液中的SDF-1α含量,分析其在不同分型的急性白血病中的变化及其临床意义。方法选择急性白血病患者作为实验组,同期以颅脑外伤患者作为对照组。实验组采用标准方案化疗2周期。采集2组患者治疗前后的静脉血及脑脊液标本,采用酶联免疫吸附法(ELISA)检测入组患者血清及脑脊液中的SDF-1α含量。结果治疗2周期后,实验组患者血清及脑脊液SDF-1α含量均低于治疗前(P<0.05)。治疗前后实验组与对照组比较,血清及脑脊液SDF-1α含量均明显升高(P<0.05)。与AML组比较,ALL组在治疗前后的血清及脑脊液SDF-1α含量较高;与非CNSL患者比较,CNSL组SDF-1α含量升高(P<0.05)。治疗2周期后,疗效为CR的患者SDF-1α含量较NR患者明显降低(P<0.05)。结论 SDF-1α与急性白血病的中枢神经系统浸润有关,在血清及脑脊液中含量变化可作为病情严重程度评估及预后判断的指标。

Objective To detect SDF-1α in serum and cerebrospinal fluid of acute leukemia patients,and analyze its change in different classification of acute leukemia and clinical significance. Methods Patients with acute leukemia was the ex- perimental group, at the same time craniocerebral trauma patients was control group. The experimental group received 2 cycles of chemotherapy with a standard solution. Venous blood and cerebrospinal fluid specimens were collected in the 2 groups before and after treatment, and SDF-1α content in serum and cerebrospinal fluid were detected using enzyme-linked immunosorbent （ELISA）. Results after 2 cycles, serum and cerebrospinal fluid SDF-1α levels in the experimental group were lower than before treatment（ P 〈 0.05 ） ;Before and after treatment compared with the control group, serum and cerebrospinal fluid SDF-1α levels in the experimental group were significantly higher（ P 〈 0.05 ） ;Compared with AML group, serum and cerebrospinal fluid SDF-1α content in ALL group before and after treatment was higher, compared non-CNSL, contents of SDF-1α in CNSL increased （P 〈 0.05 ） ; after 2 cycles,the SDF-1α content in CR patients was significantly lower than NR group （ P 〈 0.05 ）. Conclusion SDF- 1α is associated with the central nervous system infiltration of acute leukemia, content changes in serum and cerebrospinal fluid can be used as indicators to illness severity evaluation and prognosis.