高度近视患者血清中MMP-2和TIMP-2的含量与眼轴长度的相关性（英文）

MMP-2 and TIMP-2 levels in human serum:relationship with axial length

目的：检测高度近视患者血清中MMP-2和TIMP-2的含量并探究它们与眼轴长度之间的相关性。方法：选取2015-05/2015-12在武警总医院就诊的13例高度近视患者为观察组,并以同期的13例性别、年龄相匹配的正常眼轴患者为对照组。用A超测量眼轴长度,ELISA检测两组患者血清中MMP-2及TIMP-2含量,并比较两检测指标与眼轴长度值之间的相关关系。结果：高度近视组血清中MMP-2和TIMP-2的含量均低于对照组（分别为P_1=0.027和P2=0.048）。MMP-2含量在高度近视组为10.21（9.26~11.49）pg/mL,而在对照组为11.56（11.03~15.14）pg/mL;TIMP-2含量在高度近视组为79.69（72.86~93.89）pg/mL,而在对照组中为93.16（87.54~100.97）pg/mL。TIMP2/MMP2两组患者之间差异无统计学意义（P=0.216）。血清中MMP-2及TIMP-2含量均与眼轴长度呈负相关（r_1=-0.512,P1=0.007;r_2=-0.604,P_2=0.001）;TIMP2/MMP2与眼轴长度之间的相关性无统计学意义（r=0.385,P=0.052）。结论：血清中MMP-2及TIMP-2的含量随眼轴的增加而降低,且均与眼轴长度呈负相关。

AIM：To assess the serum levels of matrix metalloproteinase 2（MMP- 2） and tissue inhibitor of matrix metalloproteinases 2（TIMP-2） in patients with high myopia（HM）,and to investigate the relationship between their levels and axial length（AL）.METHODS：HM patients（13 eyes）（group A） and agematched healthy volunteers with normal axial length eyes（13 eyes）（group B） who acted as controls were recruited.Blood samples were collected from all subjects in the early morning hours after an overnight fast.MMP-2 and TIMP- 2 levels were measured by specific immunoassays（enzyme-linked immunosorbent assay）.RESULTS：The serum MMP-2 and TIMP-2 levels were decreased in the eyes of patients with HM compared with those of controls（P=0.027 and P=0.048,respectively）.The median MMP-2 level was 10.21 pg/ml（range,9.26-11.49 pg/ml） in the group A and 11.56 pg/ml（range,11.03-15.14 pg/ml） in the control group.The median TIMP-2 level was 79.69 pg/ml（range,72.86-93.89 pg/ml） in the group A and 93.16 pg/ml（range,87.54-100.97 pg/ml） in the control group.There was no significant difference between the two groups in TIMP2/ MMP2（P=0.216） ratio.MMP-2 and TIMP-2 levels were negatively correlated with AL（r_1 =-0.512,P_1 = 0.007;r_2 =-0.604,P_2 = 0.001）.No correlation was observed between the ratio TIMP2/ MMP2 and AL（r =0.385,P= 0.052）.CONCLUSION：This pilot study reveals that the levels of MMP-2 and TIMP-2 could be detected in the serum.The levels of serum MMP-2 and TIMP-2 were decreased in the eyes with elongated axis,and their levels were nevatively correlated with AL.