摘要
目的探讨糖尿病肾病(DN)患者肾小球内10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)及结蛋白(desmin)表达的意义。方法经临床和病理证实为DN患者的肾组织30例,根据肾小球病变分为Ⅰ级病变组、Ⅱ级病变组和Ⅲ级病变组,10例肾肿瘤患者远端正常肾组织作为对照。采用免疫组织化学方法检测各组肾小球内PTEN及desmin的表达。结果随着肾小球病变加重,肾小球内desmin表达逐渐增多,PTEN表达逐渐减弱。肾小球内PTEN表达与24h尿蛋白水平和desmin的表达呈负相关(r=-0.675、-0.642,P均<0.01)。结论 PTEN表达下调可能介导了DN的足细胞损伤,加剧DN的进展。
Objective To investigate the role of PTEN and desmin in glomeruli in patients with diabetic nephropathy （DN）. Methods Thirty patients diagnosed as diabetic nephropathy by clinical data and renal biopsy were divided into three groups （grade I , II , III ） by glomerular lesion, 10 cases of renal tissues without evident renal disease were obtained from distant portion of renal tumors as control group. The expression of PTEN and desmin in glomeruli were detected by immunocytochemistry. Results Immunocytochemical staining showed that the expression of desmin strengthened with the severity of the lesions of glomeruli in DN, but the expression of PTEN was less remarkable. There were obviously negative correlation between the expression of PTEN in glomeruli and 24 h urine protein excretion and the expression of desmin in glomeruli （ r = -0. 675 and -0. 642, respectively, P 〈0.01） . Conclusion The downregulation of PTEN expression may lead to podocyte lesion and aggravate progress of diabetic nephropathy.
出处
《临床荟萃》
CAS
2016年第8期897-900,共4页
Clinical Focus
基金
河北省卫生厅医学科学研究重点课题(20110081)
