苹果园鳞翅目夜蛾科DNA条形码鉴定

DNA barcoding of moths( Lepidoptera: Noctuidae) in an apple orchard

为了检验DNA条形码在鳞翅目夜蛾科蛾类鉴定中的可行性,本文对采自北京昌平苹果园内的夜蛾科14种71头蛾类标本分别提取了DNA,并扩增了线粒体cox1及核基因28S,利用系统发育树、遗传距离、阈值等方法进行了鉴定和比较分析。同时,检验了目前BOLD系统的鉴定成功率。实验表明,基于cox1基因和BOLD系统的鉴定成功率达到了100%,而基于28S则很低,为64.8%。用不同方法构建的系统发育树,鉴定结果均相同。93%的种内遗传距离小于1%,94%的种间遗传距离为大于3%,种内种间的遗传距离形成明显的3%阈值现象。

In order to test the feasibility of DNA barcoding in identification of noctuid moths of Lepidoptera,71 samples（ 14 species in total） were collected from an apple orchard of Changping District,Beijing. And the mitochondrial gene of cox1 and the nuclear gene of 28 S were amplified and sequenced.The comprehensive analysis was performed with the methods of phylogenetic trees,genetic distance,and threshold value. Meanwhile,the success rate of BOLD system for identifying species was examined. The results were as follows： With the cox1 gene and the BOLD system,the success rate of identification achieved 100%. However,the fragment of 28 S only possessed the success rate of 64. 8%. Using different methods of phylogenetic analysis,the topology structures of different phylogeny trees were consistent,as was the case in species identification. The intraspecific distances that below 1% accounted for 93%,and the interspecific distances that more than 3% occupied 94%. The interspecific genetic distances were greater than the intraspecific distances,with the threshold value of 3%.