肉桂醛对db/db小鼠胰腺抗氧化能力的影响

Effect of Cinnamic Aldehyde on the antioxidant activity in pancreas of db/db mice

目的观察肉桂醛(CA)对db/db小鼠胰腺细胞的抗氧化活性的影响,并探讨其降糖机制。方法将21只db/db小鼠随机分为对照组(Con)、CA低剂量组、CA高剂量组,分别予安慰剂,CA低剂量和CA高剂量。灌胃3周后,检测各组血糖、血清和胰腺组织抗氧化酶活性及胰腺细胞线粒体活性等。结果与Con组比较,CA低剂量和CA高剂量组血糖降低(P<0.05);CA高剂量组胰腺抗氧化酶的活性均增强,胰腺细胞线粒体活性氧簇(ROS)量降低(P<0.05);CA低剂量组ROS量与Con组比较,差异无统计学意义(P>0.05)。此外,给药组线粒体膜电位均高于Con组,CA高剂量组琥珀酸脱氢酶(SDH)活性增加(P<0.05)。结论 CA能降低db/db小鼠血糖,其作用机制是通过提高胰腺组织抗氧化酶活性,减少体ROS等自由基的产生,从而保护线粒体功能,保护胰腺细胞。

Objective To investigate the effect of cinnamic aldehyde （CA） on the antioxidant activity in pancreas of db/db mice and its mechanism of reducing blood glucose. Methods 21 db / db mice were randomly divided into model group （Con） treated with placebo intragastric administration, low dose CA group and high dose CA group. After 3 weeks, blood glucose, antioxidant enzyme activity in pancreatic tissue and mitochondrial activity in pancreatic cells were detected and compared among three groups. Results The two groups treated with CA had lower levels of glucose than Con group. Compared with Con group, antioxidant enzyme activities were higher and mitochondrial reactive oxygen species （ROS） were lower in high dose CA group（P〈0.05） ;but no significant difference was found in low dose CA group （P〉 0.05）. Mitochondrial membrane electric potentials were higher in the two CA treatment groups than in Con group. The succinate dehydrogenase （SDH） activity in high dose CA group was significantly increased （P〈 0.05）. Conclusion Cinnamic aldehyde can reduce blood glucose by increasing the activity of antioxidant enzyme and reducing the activity of ROS in pancreas of db / db mice.