TNBS模型大鼠结肠组织中NF-κB、PPAR-γ的表达特点

The Expression Characteristics of NF-κB and PPAR-γ in Colon Tissue of TNBS Rats

目的观察三硝基苯磺酸（TNBS）造模后第3日、7日、10日、14日大鼠结肠组织中核转录因子-κB（NF-κB）、过氧化物酶体增殖物激活受体-γ（PPAR-γ）表达的变化特点。方法造模组采用TNBS/乙醇溶液灌肠法制作UC大鼠模型,将大鼠分为空白组、造模后3 d组、造模后7 d组、造模后10 d组、造模后14 d组。后4组分别于造模后第3日、7日、10日、14日处死,采用免疫组化法及实时荧光定量PCR法测定各组大鼠结肠组织中NF-κB、PPAR-γ及m RNA表达。结果造模后3 d大鼠结肠NF-κB表达显著上调,10 d、14 d后表达较前逐渐回落;造模后3 d大鼠结肠PPAR-γ表达明显下调,至14 d时其表达逐渐恢复。结论 TNBS大鼠结肠炎是炎症性肠病的良好模型,造模后结肠NF-κB表达上调,PPAR-γ表达受抑制,在造模后14 d后模型自愈较为明显,治疗的疗程选择7~10 d为宜。

Objective：To observe the expression characteristics of NF-κB and PPAR-γ in colon tissue of TNBS rats after successful modeling on 3rdday,7thday,10 thday and 14 thday.Methods：The models were made with TNBS/ethanol solution enema method.The rats were divided into the blank group,3 d group,7 d group,10 d group and 14 d group.The latter four groups were killed according to time.The expression of NF-κB,PPAR-γand m RNA was detected by immunohistochemistry and real-time fluorescence quantitative PCR method.Results：3 days after modeling,NF-κB expression of rat colon was significantly up-regulated,but after 10 days and 14 days,the expression gradually declined.3 days after modeling,PPAR-γ expression of rat colon significantly reduced.Till 14 thday,the expression gradually restored.Conclusion：TNBS colitis in rats is a good model of IBD.After modeling,NF-κB of colon up-regulated and PPAR-γ expression was inhibited.14 d after modeling,self-healing of model is more obvious,and the course of treatment lasting 7 to 10 days is appropriate.