银杏叶提取物对肠缺血再灌注肝损伤的保护作用

Protective Effects of Ginkgo Biloba Extract on Intestinal Ischemia-reperfusion Injury in the Liver

目的观察银杏叶提取物(EGb)对大鼠肠缺血再灌注肝损伤的保护作用。方法选择健康雄性SD大鼠32只,随机分为4组,每组8只。分别为正常组、模型组、EGb小剂量组、EGb大剂量组。检测血清中ALT和AST,观察肝组织病理学改变,用Western blot检查肝组织芳香烃受体(AhR)。结果大鼠在肠缺血再灌注后,血清中ALT和AST含量显著升高;肝组织中AhR的表达量显著增加;光镜下,肝组织的中央静脉和肝血窦淤血严重,局部坏死。EGb大剂量组与模型组比,ALT和AST水平明显下降,AhR的表达明显受到抑制,肝组织的淤血情况明显减轻。结论银杏叶提取物对肠缺血再灌注肝损伤有保护作用,其可能的机制是通过抑制AhR的表达来发挥作用。

Objective To investigate the protective effect of Ginkgo biloba extract on intestinal ischemia-repeifusion injury in the liver. Methods Healthy male SD rats were randomly divided into four groups ： Normalgroup,model group, EGb low-dose group, and EGb high-dose group. The levels of serum alanine aminotransferase（ALT） and aspartate transaminase （AST） were determined. The microscopical structure of the liver tissuewas observed by HE staining. The expression of AhR was determined by Western blot. Results After intestinalischemia and reperfusion, the serum ALT and AST significantly increased in rats and the expression of liver tissueof AhR increased significantly. Under the light microscope, the central vein and hepatic blood sinus congestionof liver tissue were serious and local tissue became necrosis. Compared with the model group,the levels of ALT and AST decreased significantly, AhR expression was significantly inhibited and congestion of liver tissuesignificantly reduced in EGb high dose group. Conclusion Ginkgo biloba extract has a protective effect on theliver injury of intestinal ischemia-repeifusion in rats,and its possible mechanism is by inhibiting the expressionof AhR .