新生儿肺炎患儿痰细菌培养检测及耐药分析

Sputum bacterial culture detection and drug resistance analysis of neonatal pneumonia

目的:观察新生儿肺炎患儿痰病原菌的种类及耐药情况。方法:选取新生儿肺炎的患儿182例进行负压吸痰;将痰液细菌进行培养及药物敏感试验,并对常见病原微生物进行病原学分析。结果:182例新生儿肺炎的患儿痰液培养中均为阳性。常见的细菌为大肠埃希菌(31.3%)、肺炎克雷伯菌(26.9%)、金黄色葡萄球菌(8.2%)、流感嗜血杆菌(7.7%)、阴沟肠杆菌(7.1%)、肺炎链球菌(1.6%)、卡塔莫拉菌(2.2%)。药物敏感试验显示:大多细菌对青霉素耐药,对亚胺培南,美诺培南及万古霉素敏感。大肠埃希菌和肺炎克雷伯菌ES-BLs的产生率高。结论:大肠埃希菌,肺炎克雷伯菌,金黄色葡萄球菌,流感嗜血杆菌是新生儿肺炎患儿主要病原菌,对青霉素普遍耐药。

Objective To investigate the types and drug resistance of pathogenic bacteria in neonatal pneumonia. Methods：182 children with neonatal pneumonia were selected and the sputum specimens were sampled by negative pressure suction. The sputumbacterial culture and drug sensitivity tests were performed, and the common pathogenic microorganisms were pathogenically analyzed . Results： The results of the sputum bacterial culture of the 182 neonates were all positive, and the common bacteria wereEscherichia coli （31. 3 % ）, Klebsiella pneumoniae （26. 9% ） , Staphylococcus aureus （8. 2% ） , Haemophilus influenzae （7. 7 % ）,Enterobacter cloacae （7. 1 % ）, Streptococcus pneumoniae （ 1. 6% ） , and Moraxella catarrhalis （2. 2 % ）. The drug sensitive testshowed that most bacteria were resistant to Penicillin, Imipenem, Meropenem and Vancomycin. The production rate of Escherichia coliand klebsiella pneumoniae ES-BLs was high. Conclusions： Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, andHaemophilus influenzae are the main pathogenic bacteria of neonatal pneumonia, and resistant to Penicillin.