miR-125a-5p对胰腺癌细胞增殖、凋亡和细胞周期的影响

Effects of miR-125a-5p on Cell Proliferation,Apoptosis and Cell Cycle of Pancreatic Cancer Cells

目的探讨miR-125a-5p对胰腺癌细胞增殖、凋亡和细胞周期的影响。方法荧光定量PCR检测胰腺癌组织中miR-125a-5p的表达水平,细胞计数试剂盒-8检测下调miR-125a-5p水平对胰腺癌细胞生长的影响,流式细胞术检测下调miR-125a-5p表达水平对胰腺癌细胞周期和凋亡的影响,软琼脂集落形成实验评价miR-125a-5p在胰腺癌细胞恶性转化过程中的作用。结果 miR-125a-5p在胰腺癌组织的表达高于癌旁正常组织(P<0.05)。下调miR-125a-5p表达水平后,胰腺癌细胞系Panc-1和MIA Pa Ca-2的生长受到抑制(P<0.05),早期凋亡率分别增加13.6%和11.0%(P<0.05),细胞集落数目分别下降27.3%和27.8%(P<0.05),Panc-1细胞S期细胞百分比降低11.8%(P<0.05)。结论 miR-125a-5p在胰腺癌组织中高表达,下调miR-125a-5p表达水平使胰腺癌细胞生长受到抑制,集落形成能力下降,细胞周期受到阻滞,凋亡比例增加,提示miR-125a-5p在胰腺癌中发挥癌基因的作用。

Objective To investigate the effects of miR-125a-5p on cell proliferation,apoptosis and cell cycle of pancreatic cancer cells. Methods The expression level of miR-125a-5p in pancreatic cancer was determined using quantitative real-time polymerase chain reaction analysis in 4 pairs of pancreatic cancer tissues and matched adjacent normal tissues samples. The expression of miR-125a-5p was downregulated in pancreatic cancer cell lines by transfection with miR-125 a-5 p inhibitor. Cell counting kit-8 assays was conducted to detect the growth ability of pancreatic cancer cell lines. Flow cytometry was applied to detect the cell cycle and apopotosis. Soft agar colony formation test was employed to assess the role of miR-125 a-5 p in process of malignant transformation. Results MiR-125 a-5 p was significantly highly expressed in pancreatic ductal adenocarcinoma tissues than adjacent normal tissues（ P〈0. 05）. After the expression level of miR-125 a-5 p in Panc-1and MIA Pa Ca-2 was downregulated,the growth ability was suppressed（ P〈0. 05）,early apopotosis rate was promoted by 13. 6% and 11. 0% respectively（ P〈0. 05）, the amount of colony formation was reduced by27. 3% and 27. 8%,respectively（ P〈0. 05）,and the percentage of S stage of Panc-1 was reduced by 11. 8%（ P〈0. 05）. Conclusions The expression of miR-125a-5p is high in pancreatic ductal adenocarcinoma tissues. After the expression level of miR-125a-5p is downregulated,the growth ability,colony formation,and cell cycle of Panc-1 and MIA Pa Ca-2 are suppressed,and the early apopotosis rate will be promoted. Therefore,miR-125a-5p may play an oncogenic role in pancreatic ductal adenocarcinoma.