乙酰甲喹影响猪尿液代谢组的NMR研究

Effects of Acute Mequindox Exposure on Urinary Metabonome in Pigs

乙酰甲喹是喹噁啉类广谱抗菌药,在我国畜禽养殖业中有广泛的应用.乙酰甲喹的毒性以及毒性机制被越来越多的报道,然而,乙酰甲喹对动物内源性代谢的影响仍不清楚.该研究利用基于核磁共振(NMR)的代谢组学方法并结合多变量数据分析方法研究了乙酰甲喹一次性急性给药(10 mg/kg.bw)对猪尿液代谢组的影响.结果发现,乙酰甲喹给药后,尿液中肌酸、马尿酸和对甲酚葡萄糖醛酸浓度显著降低,而对甲基苯酚和苯甲酸的浓度在给药后显著升高.乙酰甲喹对猪尿液代谢组的影响在给药144 h后未见恢复.研究结果显示,剂量为10 mg/kg.bw乙酰甲喹给药主要影响了猪体内肠道菌群微生态.上述研究为乙酰甲喹对生物体内源性代谢的影响提供了基础数据,并为乙酰甲喹的安全用药提供了数据参考.

Mequindox is a member of the quinoxaline family, and widely used in Chineseveterinary industry as an antibacterial drug. Toxicity of mequindox and the underlyingmechanisms have been studied previously. However, endogenous metabolic alterations inanimals caused by mequindox exposure are currently unknown. This study investigated theeffects of acute mequindox exposure （10 mg/kg body weight） on urinary metabonome inpigs using an NMR-based metabonomics approach. The results showed that acutemequindox exposure significantly decreased the levels of creatine, hippurate and p-cresolglucuronide in the urine of treated pigs, whereas the levels of p-cresol and benzoateincreased significantly. The changes of urinary metabolic profiles were long-lasting, anddid not recover at 144 h post-exposure. At the dose given, mequindox mainly causeddisturbance of gut microbial ecosystem. This study provided useful information on theeffects of mequindox on metabolism in pigs, and might guide the safe application ofmequindox in veterinary industry.