Gli1在食管鳞状细胞癌中的表达及与预后的关系

The expression of Gli1 and its prognostic implication in patients with esophageal squamous cell carcinoma

目的:探讨Hh信号通路关键因子Gli1在食管鳞状细胞癌(ESCC)中的表达及临床意义,并分析其与预后的关系。方法:食管鳞状细胞癌组织及癌旁正常食管组织各88例,采用免疫组织化学法检测Gli1的表达,另用Western-blot方法检测其中30例ESCC的Gli1的表达,Pearson卡方检验分析Gli1表达与临床病理特征之间的关系。单因素Kaplan-Meier法分析Gli1表达与预后的关系。Cox比例风险回归评估Gli1表达与预后的关系。结果:食管鳞状细胞癌组织中Gli1的表达在蛋白水平明显高于食管正常鳞状上皮,Gli1在ESCC中免疫组化阳性表达率为71.59%(63/88),Gli1在癌旁正常食管鳞状上皮中免疫组化阳性表达率为6.82%(6/88),差异有统计学意义(P<0.05)。Western blot法证实Gli1蛋白在ESCC组织中表达上调,与癌旁正常食管组织中的表达比较,差异有统计学意义(P<0.001)。Gli1高表达与肿瘤病理分期及淋巴结转移密切相关(P<0.05)。单因素分析Kaplan-Meier法提示Gli1阳性表达组与阴性表达组生存率之间的差别有统计学意义(Log-rank test,P=0.005)。结论:食管鳞状细胞癌中Gli1的表达比较普遍,与ESCC的预后密切相关,可作为预测转移潜能和预后判断的有价值的指标。

Objective： To evaluate the expression of Gli1 and its prognosis implication in patients with esophageal squamous cell carcinoma（ ESCC）. Methods： Tissue specimens were collected during thoracic surgery from 88 patients and were divided into two groups： the ESCC tissues group and the adjacent normal esophageal tissue group. Gli1 protein expression was detected by immunohistochemistry（ IHC） and Western blot. The relationship between Gli1 and clinicopathological parmeters and patients＇ prognosis was analyzed by Chi- square test and COX proportional hazards regression model. Results： Immunochemistry showed in 88 ESCC tissue slices,the positive ratios of Gli1 was 71. 59%（ 63 /88）,whereas in the adjacent normal esophageal mucosa,the positive ratios of Gli1 was 6. 82%（ 6 /88）. Western- blot analysis showed that the expression of Gli1 was undetected in the adjacent normal esophageal mucosa,whereas the positive expression of Gli1 was detected in most ESCC. The expression of Gli1 was higher in ESCC than in the adjacent normal esophageal mucosa（ P〈0. 05）. The expression of Gli1 was significantly correlated with lymph node metastasis and TNM stages（ P〈0. 05）. The expression of Gli1 was an independent prognostic indicator for the overall survival of patient positive in ESCC（ Log- rank test,P = 0. 005）. Conclusion： Currently as the evaluating indicator of lymph node metastasis activation,the Gli1 generally expressed and associated with prognostic implication was elevated in ESCC.