黄瓜绿斑驳花叶病毒安徽分离物全基因组序列测定及分析

Cloning and sequence analysis of the genome of Cucumber green mottle mosaic virus Anhui isolate

通过胶体金免疫层析试纸条和RT-PCR等手段对采自安徽和县的西瓜病株进行检测,确定其病原为黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)。为明确CGMMV安徽分离物CGMMV-Anhui的分类地位,进一步克隆了该病毒的全基因组序列,分析了其基因组结构特征。结果表明,CGMMV-Anhui基因组全长为6 423bp(GenBank登录号KT236095),与已报道的CGMMV的编码区的基因结构一致,仅5′和3′端非编码区核苷酸数目略有差异。将CGMMV-Anhui与已报道的分离物的全基因组序列和外壳蛋白基因序列进行系统发育分析,显示CGMMV不同分离物可分为亚洲和欧洲两个组,安徽分离物CGMMV-Anhui与亚洲分离物亲缘关系较近,可能具有共同的侵染源。

Based on gold immunochromatographic assay and RT-PCR methods, the pathogen infecting watermelon in He County of Anhui Province was identified as Cucumber green mottle mosaic virus （CGMMV）. In order to clarify the genome characteristics of Cucumber green mottle mosaic virus isolate from Anhui （CGMMV-Anhui）, the complete genomic sequence of CGMMV-Anhui was determined by molecular cloning. The results showed that the genome of CGMMV-Anhui contained 6 423 nucleotides （GenBank accession no. KT236095） and its genomic structure was the same as that of the CGMMV isolates previously reported, except for the nucleotide numbers of 5＇-noncoding region （NCR） and 3＇-NCR. The phylogenetic relationship of CGMMV-Anhui with other reported isolates was analyzed based on the genomic sequence and coat protein gene sequence. The analysis of phylogenetic tree showed that CGMMV could be separated into Asian isolates and European isolates. CGMMV-Anhui showed the closest relationship with Asian isolates and they might share the same infection source.