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大豆主要过敏原Gly m Bd 28K的同源建模和B细胞表位的预测 被引量：9

HOMOLOGOUS MODELING AND PREDICTION OF B CELL EPITOPE OF SOYBEAN MAJOR ALLERGEN GLY M BD 28K
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摘要 利用生物信息学软件SWISS-MODEL和Deep View 4.1对Gly m Bd 28K进行同源建模并对做出的模型进行评估。利用DNAStar和SOPMA分析Gly m Bd 28K的亲水性、柔韧性、表面可及性、抗原指数和二级结构,预测出Gly m Bd 28K的4个B细胞线性表位5-13,75-77,175-177,200-203。根据三级结构模型,通过Disco Tope 2.0预测出Gly m Bd 28K的6个与构象表位相关的区段11-14,40-41,43,114-115,185-192,194-207。 The model of tertiary structure of Gly m Bd 28 K was constructed by the bioinformatics tool SWISS-MODEL,and its reliability was evaluated by Deep View 4.1. DNAStar and SOPMA were used to analyze the hydrophilicity,flexible region,surface probability,antigenic index and secondary structure of Gly m Bd 28 K protein,and based on that,four liner epitopes of B cell including 5-13,75-77,175-177 and 200-203 were predicted. According to the tertiary structure,six regions related to the conformational epitopes of Gly m Bd 28 K including 11-14,40-41,43,114-115,185-192,194-207 were predicted using software Disco Tope 2.0.
作者 闫慧丽 席俊 贺梦雪 皮江一 陆启玉
机构地区 河南工业大学粮油食品学院
出处 《河南工业大学学报（自然科学版）》 CAS 北大核心 2016年第4期41-45,51,共6页 Journal of Henan University of Technology：Natural Science Edition
基金 国家自然科学基金项目(31301409) 河南工业大学自然科学基础研究计划(2014JCYJ01)
关键词 GLY M BD 28K 三级结构 B细胞表位 生物信息学软件 Gly m Bd 28K tertiary structure B cell epitope bioinformatics tool
分类号 TS201.2 [轻工技术与工程—食品科学]
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参考文献13

  • 1李平,王转花.Cupin家族食物性过敏原表位研究进展[J].细胞与分子免疫学杂志,2011,27(5):590-592. 被引量：9
  • 2KUMARI D,ARORA N,KASERA R,et al.Isolation and characterization of a 28 k Da major allergen from blackgram(Phaseolus mungo)[J].Immunobiology,2012,217(9):895-904.
  • 3SORIA-GUERRA R E,NIETO-GOMEZ R,GOVEA-ALONSO D O,et al.An overview of bioinformatics tools for epitope prediction:Implications on vaccine development[J].J Biomed Inform,2015,53:405-414.
  • 4JIN T,WANG Y,CHEN Y W,et al.Crystal structure of Korean pine(Pinus koraiensis)7S seed storage protein with copper ligands[J].J Agric Food Chem,2014,62(1):222-228.
  • 5CARUGO O,DJINOVIC-CARUGO K.A proteomic Ramachandran plot(PRplot)[J].Amino Acids,2013,44(2):781-790.
  • 6DEL魪AGE G,ROUX B.An algorithm for protein secondary structure prediction based on class prediction[J].Protein Eng,1987,1(4):289-294.
  • 7KRINGELUM J V,LUNDEGAARD C,LUND O,et al.Reliable B cell epitope predictions:impacts of method development and improved benchmarking[J].PLo S Comput Biol,2012,8(12):1776.
  • 8SAHA S,RAGHAVA G P S.Prediction of continuous B-cell epitopes in an antigen using recurrent neural network[J].Proteins-structure Function&Bioinformatics,2006,65(1):40-48.
  • 9LARSEN J E P,LUND O,NIELSEN M.Improved method for predicting linear B-cell epitopes[J].Immunome Res,2006,2(1):2.
  • 10SUN X,SHAN X,YAN Z,et al.Prediction and characterization of the linear Ig E epitopes for the major soybean allergenβ-conglycinin using immunoinformatics tools[J].Food Chem Toxicol,2013,56(12):254-260.

二级参考文献20

  • 1Rabjohn P, Helm EM, Stanley JS, et al. Molecular cloning and epitope analysis of the peanut allergen Ara h 3 [ J ]. J Clin Invest, 1999, 103 : 535 -542.
  • 2Shin DS, Compadre CM, Maleki SJ, et al. Biochemical and structur- al analysis of the IgE binding sites on Ara hi, an abundant and high- ly allergeic peanut protein[J]. J Biol Chem, 1998, 273:13753 - 13759.
  • 3Stanley JS, King N, Burks AW, et al. Identification and mutational analysis of the immunodominant IgE binding epitopes of the major peanut allergen Ara h 2[J]. Arch Biochem Biophys, 1997, 342: 244 - 253.
  • 4Bane A, Sordet C, Culerrier R, et al. Vicihn allergens of peanut and tree nuts (walnut, hazelnut and cashew nut) share structurally related IgE-binding epitopes[ J]. Molecular Immunology, 2008, 45 (5) : 1231 -1240.
  • 5Restani P, Ballabio C, Corsini E, et al. Identification of the basic subunit of Ara h 3 as the major allergen in a group of choldren aller- gic to peanuts[ J]. Ann Allergy Asthma Immunol, 2005, 94:262 - 266.
  • 6Pierre R, Rapha C, Virginie S, et al. Mapping and conformational analysis of IgE-binding epitopic regions on the molecular surface of the major Ara h 3 legumin allergen of peanut (Arachis hypogaea) [ J]. Molecular Immunology, 2009, 46 : 1067 - 1075.
  • 7Jason MR, Gregg GH, Suzanne ST, et al. Linear IgE-epitope map- ping and comparative structural homology modeling of hazelnut and English walnut 11S globulins [ J ]. Molecular Immunology, 2009, 46 : 2975 - 2984.
  • 8Dunwell JM. Cupins: a new superfamily of functionally diverse pro- teins that include germins and plant storage proteins [ J ]. Biotechnol Genet Eng Rev, 1998, 15:1-32.
  • 9Dunwell JM, Purvis A, Khuri S. Cupins: the most functionally diverse protein superfamily[ J]. Phytochemistry, 2004, 65: 7-17.
  • 10Christian R, Heimo B. Evolutionary biology of plant food allergens [J]. J Allergy Clin Immunol , 2007, 120:518 -525.

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河南工业大学学报（自然科学版）
2016年 第4期

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