摘要
目的用紫外分光光度法测定刺芫婪中总黄酮的含量。方法用60％的乙醇提取总黄酮，以芦丁为对照品，在紫外510nm处测定。结果芦丁检测浓度在0．0128—0．064mg／L范围内与吸光度呈现良好的线性关系，R^2=0．9996，加样平均回收率101．69％，RSD=0．57％（n=6）；用乙醇回流提取法提取的总黄酮含量可达到1．079％。结论本方法简便、快速、准确，操作简单易行，适用于黄酮类物质含量的测定。
Objective： To determine total flavonoids in Eryngium foetidum Linn by UV-spectrometry. Methods： Total flavonoids was extracted with 60 % ethanol and rutin was used as reference. The content was detected at 510 nm. Results： Calibration graphs were constructed in the range of 0.0128-0.064 mg/mL for total flavonoids, R^2=0.9996. The averager ecovery was 101.69 % for total flavonoids. The RSD of the method was 0.57 %, and the content of total flavonoids was 1.079 %. Conclusion： The method was proved to be simple, feasible, fast, and can be used for quality control for flavonoids in Eryngium foetidum Linn.
出处
《广东化工》
CAS
2016年第16期173-174,共2页
Guangdong Chemical Industry
基金
海南省大学生创新项目(20150072)
海南医学院培育基金项目(HY2014-21)
关键词
刺芫荽
分光光度法
总黄酮含量
Eryngium foetidum Linn
spectrophotometry
total flavonoids
