两种样品处理方法对LC-MS/MS测定鸡肉中金刚烷胺基质效应比较

Comparasion of Two Pretreatment Methods on the Matrix Effects for the Determination of Amantadine in Chicken by Liquid Chromatography-Tandem Mass Spectrometry

比较了液液萃取结合分散固相萃取(QuEChERS)和Oasis MCX小柱固相萃取(SPE)提取两种样品处理方法,分析鸡肉中金刚烷胺的基质效应。从绝对基质效应、提取回收率、方法过程效率及方法稳定性等方面的考察发现,经QuEChERS法和SPE法两种前处理方法处理后,不同浓度金刚烷胺的绝对基质效应均大于90%。SPE法虽能增强金刚烷胺的离子化效率,但金刚烷胺的提取回收率小于85%,方法过程效率也很低。与之相比,采用QuEChERS法处理鸡肉样品后,不仅能够有效增强金刚烷胺离子化效率,同时提取回收率和方法过程效率也相对较高且稳定,更适合作为鸡肉中金刚烷胺检测的前处理方法。

This paper focused on the comparision of matrix effects for the determination of concentration of amantadine in chicken by two different pretreatment methods QuEChERS and solid phose extraction（SPE）. The absolute matrix effects, the recoveries, the process efficiencies and the method stability of two methods were evaluated. The results showed that the absolute matrix effects of two methods were all above 90%. After the sample pretreatment of SPE method, the ionization efficiency of amantadine could be enhanced, however, the recoveries of amantadine in chicken were below 85%, and the process efficiencies were also quite low. Compared with the SPE method, the ionization efficiencies of amantadine could enhance effectively after QuEChERS pretreatment,furthermore,the extraction recoveries and process efficiencies were also relatively higher and more stable. So the QuEChERS method was better as a pretreatment technique for the determination of amandine in chicken.