蓝玉簪龙胆黄酮苷类化学成分研究

Study on flavonoid glycosides from Gentiana veitchiorum

目的研究藏药蓝玉簪龙胆Gentiana veitchiorum地上部分中的黄酮苷类化学成分。方法采用硅胶柱色谱、ODS中压柱色谱及制备高效液相色谱等方法对化合物进行分离纯化,通过1D和2D NMR方法鉴定化合物结构。结果从蓝玉簪龙胆地上部分50%乙醇提取液中分离并鉴定了6个黄酮苷类化合物,分别为5,7,3′,4′-四羟基黄酮-6-C-β-D-吡喃葡萄糖基-4′-O-β-D-吡喃葡萄糖基-(1→6)-β-D-吡喃葡萄糖苷(1)、异荭草素(2)、异牡荆苷(3)、异金雀花素(4)、异荭草素-4′-O-葡萄糖苷(5)和异皂草苷(6)。结论化合物1为新化合物,命名为异荭草素-4′-二葡萄糖苷;化合物5和6为首次从该植物中分离得到。

Objective To study the flavonoid glycosides from the aerial parts of Gentiana veitchiorum. Methods The compounds were isolated and purified by silica gel and ODS chromatography in addition to p-HPLC. The structures of the isolated compounds were identified on the basis of 1D ＆ 2D NMR spectroscopy methods. Results Six compounds were isolated from the aerial parts of G. veitchiorum and identified as 5,7,3＇,4＇-tetrahydroxyl flavone-6-C-β-D-glucopyranosyl-4＇-O-β-D-gLUCOPYRANOSYL-（1→6）-β-D-glucopyranoside （1）, isoorienfin （2）, isovitexin （3）, isoscoparine （4）, isoorientin-4＇-O-glucoside （5）, and isosaponarin （6）. Conclusion Compound 1 is a new compound named isoorientin-4＇-diglucoside, and compounds 5 and 6 are isolated from this plant for the first time.