不同产地冬凌草种质资源分子生物学分析

Molecular Analysis of Different Origin of Rabdosia Rubescens Germplasm Resources

目的：利用RAPD和ISSR分子标记技术对来自23个主要分布区的冬凌草进行遗传多样性和亲缘关系分析。方法：从48条RAPD引物中筛选出10条多态性引物,对供试材料进行PCR扩增后共获得84条条带,其中多态性条带77条（PPB=91.67%）,23份材料间的遗传相似系数（GS）为0.71~1.00;从70条ISSR引物中筛选出5条多态性引物,对供试材料进行PCR扩增后共获得21条条带,其中多态性条带16条（PPB=76.19%）,23份材料间的遗传相似系数（GS）为0.55~1.00。结果：聚类分析显示,不同地区冬凌草基因水平的分类具有较强的地域性。分布于伏牛山的冬凌草明显聚为一类,分布于太行山的冬凌草则聚为另一类。两种标记结果呈显著相关性,相关系数为0.636 5。结论：我国冬凌草植物的遗传多样性十分丰富,该研究为筛选优质种质资源提供了丰富的遗传基础。

Objective：Genetic relationship analysis from 23 main distribution area of Rabdosia rubescens by RAPD,ISSR molecular marker technology. Methods：Ten primers selected from 48 RAPD primers were used for amplification. A total of 84 hands were generated, of which 77 bands were polymorpbic bands. The percentage of polymorphic loci（PPB） was 91.67%. According to the result of RAPD amplification,the genetic similarity coefficient among 23 rabdosia rubescens accessions ranged from 0.71- 1.00. five primers selected from 70 ISSR primers were used for amplification. A total of 16 bands were generated,of which 21 bands were polymorphic bands. The percentage of polymorphic loci（PPB） was 76.19 %. Results： According to the result of ISSR amplification, the genetic similarity coefficient among 23 rabdosia rubescens accessions ranged from 0.55- 1.00. Groups cluster analysis showed that the gene level rubescens different regions with strong regional classification. Located in the Taihang Mountains rubescens significantly clustered into one group,located in Funiushan rubescens are clustered in another. The genetic similarity of two markers had a significant correlation （r = 0. 636 5）. Conclusion： Significant polymorphism and genetic diversity are observed among Rabdosia rubescens germplasm resources which could provide a wealth of genetic basis for the germplasm high quality.