微波辅助萃取十大功劳叶中的小檗碱

Microwave assisted extraction of berberine from Mahonia bealei

利用间歇式微波辅助萃取装置 ,对提取阔叶十大功劳叶中的药用有效成分小檗碱进行了研究。选择并确定了萃取液中盐酸小檗碱的分析方法 ( Al2 O3 柱层析 -紫外分光光度法 ) ;选取了甲醇、乙醇、乙醇 -水 ( V/ V=9∶ 1 )为溶剂 ;微波辐射量为 2 ,4,6min;处理比 ( g/ m L)为 1∶ 40 ,1∶ 60 ,1∶80 ;利用正交实验设计考察了不同湿分原料的微波辅助萃取效率 。

A study on microwave assisted extraction (MAE) for berberine in Mahonia bealei (Fort.)Carr. was carried out in batch equipment. The extracts were analysed at 347 nm by ultraviolet spectrophotometry. The solvent types, the intensity of microwave energy, the ratio(g·mL -1 ) of material to solvent volume were studied. The effect of microwave assisted extraction on different humidity material was investigated and optimized by means of three factor and three level factorial designs. The optimum conditions were obtained concerning different humidity material.