γ-亚麻酸菌株少根根霉原生质体形成与再生

Protoplast formation and regeneration of γ-linlenic acid producing starins of rhizopus arrhizus

用 6mg/m L纤维素酶 + 3mg/m L蜗牛酶 + 1 mg/m L溶菌酶作为混合酶酶解 2 8℃培养 2 6h的菌丝。以含 0 .6mol/L NH4Cl磷酸缓冲液 ( p H6.0 ) ,+ 0 .0 2 mol/L Mg SO4· 7H2 O+ 0 .4mmol/L Ca Cl2 作为渗透压稳定剂 ,酶解前以 0 .3% β-巯基乙醇处理 35 min,从少根根霉的菌丝中获得了大量的原生质体。同时对菌丝的培养时间、渗透压稳定剂、酶解系统和酶解温度等因素进行了观察 ,获得了制备原生质体的最适条件。

High yield of protoplasts from rhizopus arrhizus was obtained by using a combined enzyme system containing 6 mg/mL fibrin enzyme +3 mg/mL sailase +1 mg/mL lywallzyme.The effects of factors,such as mycelial ages,osmotic stabilizers,enzyme systemand temperature on the formation and regeneration of protoplast in R.arrhizus were systematically studied and optimum conditions for preparation of protoplasts of the fungus were obtained. The regeneration was tested on solid media containing osmotic stabilizer.