NOX4蛋白在乳腺癌中的表达及与^(18)F-FDG摄取值的相关性研究

Relationships between NOX4 expression and ^(18)F-FDG uptake in patients with breast cancer

目的:烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)在乳腺癌中的表达与糖代谢的相关性尚不清楚,本研究拟探讨乳腺癌^(18)F-FDG摄取值与NOX4的表达水平的相关性及其临床意义。资料和方法:本研究入组乳腺癌患者术前行氟-^(18)代脱氧葡萄糖正电子发射断层显像(18-fluorodeoxyglucose positron emission tomography,^(18)F-FDG PET)。应用免疫组织化学方法检测乳腺癌患者病灶病理标本NOX4表达。收集患者的淋巴结转移、组织分型和组织学分级病理资料,分析乳腺癌NOX4表达与^(18)F-FDG摄取值(最大标准摄取值,SUVmax)的相关性,及与肿瘤临床病理参数的关系。结果:56例患者符合入选标准而纳入研究,共56个乳腺癌灶。病灶SUVmax为4.78±2.96。NOX4表达阳性33例,阴性23例。NOX4表达阳性组SUVmax为6.42±3.23,NOX4表达阴性组SUVmax为3.22±1.57,NOX4表达阳性组的SUVmax高于表达阴性组,差异具有统计学意义(t=4.83,P<0.05)。淋巴结转移阳性组的NOX4表达阳性率高于淋巴结转移阴性组(χ~2=4.52,P<0.05)。NOX4表达与组织学类型无显著相关性(χ~2=2.88,P>0.05)。组织学分级Ⅲ级组的NOX4表达阳性率高于Ⅰ+Ⅱ级组(χ~2=6.32,P<0.01)。浸润性导管癌组SUVmax高于小叶癌组(t=2.97,P<0.05)。淋巴结转移组SUVmax高于非转移组(t=2.04,P=0.04)。组织学分级Ⅲ级组SUVmax高于Ⅰ+Ⅱ组(t=-2.5,P<0.05)。结论:乳腺癌NOX4表达水平与^(18)F-FDG摄取值具有相关性;NOX4表达、^(18)F-FDG摄取值与淋巴结转移、组织学分级具有相关性。

Objective： The relationship between the NOX4（NADPH oxidase 4） expression and glucose metabolism in breast cancer is unclear. This present study intends to study the expression levels of NOX4 and ISF-FDG uptake. And to further ex- plore the clinical significance. Methods： The patients diagnosed as breast cancer who underwent preoperative 18F-FDG PET were enrolled. We examined the maxinmm standardized uptake values（SUVmax） of lesions. The levels of NOX4 were measured with immunohistoehemical analysis. The clinical, histopathology of breast cancer patients were collected and recorded, and the correlation between NOX4 and clinical and histological predictors and metastasis were analyzed. Results： Fifty-six patients met the inclusion criteria and were included in the study, a total of 56 breast lesions. The SUVmax of lesions were 4.78±2.96. Thirty-three cases had positive expression of NOX4, 23 cases had negative expression. The SUVmax in the positive expression of NOX4 group was 6.42±3.23, the negative NOX expression group was 3.22±1.57. The SUVmax was higher in the NOX4 positive expression group than negative expression group, the difference was statistically significant （t=4.83, P〈0.05）. NOX4 ex- pression levels were significantly correlated with lymph node metastasis （X2=4.52, P〈0.05）. The expression of NOX4 and histo- logical types have no significant correlation （X2=2.88, P〉0.05）, The expression of NOX4 and histological grading were signifi- cantly related to histological grade, the positive rate of NOX4 expression in the group Ⅲ was higher than Ⅰ ＋ Ⅱ Group （X2= 6.32, P〈0.01）. The SUVmax in infiltrating ductal carcinoma was higher than lobular carcinoma （t=2.97, P〈0.05）. The SUVmax in lymph node metastasis was higher than no lymph node metastasis（t=2.04, P=0.04）. The SUVmax in the group m was high- er than Ⅰ ＋ Ⅱ Group （t=-2.5, P〈0.05）. Conclusions： The levels of NOX4 expression were correlated with the 18F-FDG uptake. The expression of NOX4 and the tSF-FDG uptake are associated with lymph node metastasis and histological grade.