舒芬太尼预先给药对浸水束缚应激大鼠胃组织ASIC3表达的影响

Effects of sufentanil pretreatments on ASIC3 expression in stomach tissues of rats with water immersion restraint stress

目的观察舒芬太尼与APETx2预先给药对浸水束缚应激(WIRS)大鼠胃组织酸敏感离子通道3(ASIC3)表达及胃黏膜损伤的影响。方法 40只雄性Wistar大鼠随机等分为对照组(C组)、应激组(W组)、舒芬太尼预先给药组(S组,腹腔内注射舒芬太尼25μg/kg)及ASIC3特异性拮抗剂APETx2预先给药组(A组,腹腔内注射APETx2 25μg/kg)。采用WIRS法复制应激性胃黏膜损伤模型,于应激6 h后留取标本测定溃疡指数(UI);黄嘌呤氧化酶法测定胃组织超氧化物歧化酶(SOD)活力与硫代巴比妥酸法测定丙二醛(MDA)含量;免疫组化和免疫印迹法测定胃组织ASIC3蛋白表达。结果与C组比较,W组、S组及A组的UI值显著升高(P均<0.01),SOD值显著下降(P均<0.01),MDA值显著升高(P均<0.01),胃组织ASIC3蛋白表达显著上调(P均<0.01)。与W组比较,S组及A组的UI值显著下降(P均<0.01),SOD值显著升高(P均<0.01),MDA值显著下降(P均<0.01);A组的ASIC3蛋白表达显著下调(P<0.01),S组差异无统计学意义(P>0.05)。结论 APETx2调控胃组织ASIC3蛋白表达,逆转氧自由基代谢失衡起胃黏膜保护作用;舒芬太尼同样通过逆转氧自由基代谢失衡起胃黏膜保护作用,但其作用机制并非通过调控ASIC3完成。

Objective To evaluate the effects of pretreatments of sufentanil and APETx2 on acid sensing ion channel 3（ ASIC3） expression in stomach tissues of rats with water immersion restraint stress（ WIRS） and gastric mucosal injury.Methods Forty male Wistar rats were randomly assigned to four groups（ n = 10 each） ： control group（ group C）,stress group（ group W）,sufentanil pretreatment group（ group S,intraperitoneal injection of 25 μg / kg sufentanil）,APETx2 pretreatment group（ group A,intraperitoneal injection of 25 μg / kg APETx2）. The stress-induced gastric mucosal injury model was established by WIRS method. Six hours after stress,the gastric tissues specimen was taken to detect the ulcer index（ UI）,the total activity of superoxide dismutase（ SOD） in gastric tissues by xanthine oxidase method,the content of malondialdehyde（ MDA） in gastric tissues by thiobarbituric acid（ TBA） method and ASIC3 protein expression in gastric tissues by immunohistochemistry and immunoblotting methods. Results Compared with group C,in groups W,S and A,the UI value increased significantly（ all P〈0. 01）; the SOD level decreased significantly（ all P〈0. 01）; the MDA level increased significantly（ all P〈0. 01）; ASIC3 protein expression in gastric tissues was up-regulated significantly（ all P〈0. 01）. Compared with group W,in groups S and A,the UI value decreased significantly（ all P〈0. 01）; the SOD level increased significantly（ all P〈0. 01）; MDA level decreased significantly（ all P〈0. 01）; ASIC3 protein expression was down-regulated significantly in group A（ P〈0. 01）,and there was no significant difference in ASIC3 protein expression between groups W and S（ P〉0. 05）. Conclusions APETx2 can play a protective role on gastric mucosa by regulating ASIC3 protein expression in gastric tissues and reversing the imbalance of oxygen free radical metabolism,while sufentanil can also play a protective role on gastric mucosa by reversing the imbalance of oxygen free radical metabolism,but its mechanism may not be through regulation of ASIC3.