慈姑活性成分对体外肝细胞损伤的保护作用

Protective effects of active part of Sagittaria Sagittifolia L. on hepatocytes injury in vitro

目的研究慈姑活性成分的保肝作用及其机理。方法采用系统化学溶剂萃取法制备慈姑不同活性成分,培养Hep G2型肝细胞,建立异烟肼和利福平合用致体外肝细胞损伤模型,给予慈姑不同活性成分处理后,利用MTT法检测肝细胞的存活率,并检测细胞培养上清液中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、乳酸脱氢酶、丙二醛的浓度。结果与模型组比较,慈姑乙酸乙酯组、慈姑石油醚组、慈姑总提取物组和慈姑水提组的细胞存活率均增加(P<0.01);慈姑石油醚组、慈姑总提取物组和慈姑水提组均可降低肝细胞培养上清液中的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、乳酸脱氢酶、丙二醛的水平(P<0.05),其中慈姑水提组效果最为明显(P<0.01)。结论提示慈姑水提部分对体外异烟肼和利福平合用致肝细胞损伤有较强保护作用,其作用可能与抗脂质过氧化有关。

Objective To study the protective effects and mechanism of active components of Sagittaria Sagittifolia L. on hepatocytes injury induced by co-administration of isoniazid and rifampin. Methods Different active ingredients of Sagittaria Sagittifolia L. were prepared by systemic chemical solvent extraction method. Hep G2 liver cells were incubated,and injured by co-administration of isoniazid and rifampin. MTT assay was used to evaluate the survival rate of liver cells after the cells had been treated with active part of Sagittaria Sagittifolia L.,culture media were collected to detected the levels of ALT,AST,LDH and MDA. Results Compared with the model group,the cell viability of ethyl acetate extract group,petroleum ether extract group,the total extract group and the water extract group was increased（ P〈0.01）; the content of ALT,AST,LDH and MDA was reduced after preventive treatment with petroleum ether extract,the total extract and the water extract,the effect of water extract group was especially obvious（ P〈0. 01）. Conclusion The water extract from Sagittaria Sagittifolia L. has strong protective effect on hepatocytes injury induced by co-administration of isoniazid and rifampin,which may be related to inhibiting the lipid peroxidation.