5种马鞭草科药用植物的抗补体活性

Anticomplementary effects of the extracts of five Verbenaceae herbs in vitro

采用细胞溶血法考察了千解草(Pygmaeopremna herbacea)、白花灯笼(Clerodendrum fortunatum L.)、尖尾枫[Callicarpa longissima(Hemsl.)Merr.]、赪桐[Clerodendrum japonicum(Thunb.)Sweet]及臭茉莉(Clerodendrum philippinum Schauer var.simplex Moldenke)等5种马鞭草科药用植物提取物经典途径和旁路途径的抗补体活性。结果表明:千解草水提取物、白花灯笼水提取物和醇提取物、尖尾枫水提取物和醇提取物、赪桐醇提取物及臭茉莉醇提取物有较强的经典途径抗补体活性,对经典途径的抗补体活性(CH50)分别为0.092±0.008,0.074±0.008,0.088±0.006,0.134±0.017,0.123±0.010,0.380±0.080及0.200±0.015 g/L;仅千解草和尖尾枫水提取物及白花灯笼醇提取物具有旁路途径抗补体活性,对旁路途径的抗补体活性(AP50)分别为0.533±0.033,0.758±0.031和0.362±0.029 g/L。因此,5种植物均具有不同程度的抗补体活性,其中白花灯笼醇提取物抗补体活性最强。

The inhibition effects of the extracts of five Verbenaceae herbs, Clerodendrumfortunatum L. , Cleroden- drum japonicum （ Thunb. ） Sweet, Clerodendrum philippinum Schauer var. simplex Moldenke, Callicarpa longissi- ma （ Hemsl. ） Merr. and Pygmaeopremna herbacea, were investigated by cell hemolysis model in vitro on classical and alternative complement activation pathways. The water extracts of Pygmaeopremna herbacea, the water extract and the ethanol extract of Clerodendrum fortunatum L., the water extract and the ethanol extract of Callicarpa longissima （ Hemsl. ） Merr. , the ethanol extracts of Clerodendrum japonicum （ Thunb. ） Sweet and Clerodendrum philippinum Schauer var. simplex Moldenke showed inhibition cell hemolysis effects On the classical path- way. Their CH50 values were 0. 092 ± 0. 008, 0. 074 ± 0. 008, 0. 088 ± 0. 006, 0. 134 ± 0. 017, 0. 123± 0. 010, 0. 380 ±0. 080, and 0. 200 ± 0. 015 g/L, respectively. The water extracts of Pygmaeopremna herbacea and Calli- carpa longissima （ Hemsl. ） Merr. and the ethanol extract of Clerodendrum fortunatum L. showed inhibition cell hemolysis effects on alternative pathway. Their AP50 values were 0. 533 ± 0. 033, 0. 758 ± 0. 031, and 0. 362 ± 0. 029 g/L, respectively. Five Verbenaceae herbs appear good anticomplementary effects in vitro. The ethanol extract of Clerodendrum fortunatum L. showed the best inhibitory activity.