雷公藤红素联合顺铂对C6胶质瘤细胞凋亡作用的研究

The investigation of the effect of Tripterine combined with Cisplatin on the apoptosis of C6 glioma cells

目的探讨雷公藤红素、顺铂联合应用对C6胶质瘤细胞的抑制效果和凋亡机制。方法雷公藤红素、顺铂单独及联合用药作用于C6胶质瘤细胞，采用CCK-8法测定各组细胞的生长抑制率，流式细胞术检测细胞凋亡情况，酶联免疫吸附实验检测细胞凋亡相关蛋白Bcl-2、Bax、XIAP、NF-κB的表达水平变化。结果相对于雷公藤红素和顺铂分别单独用药，雷公藤红素能够协同顺铂显著抑制C6胶质瘤细胞地生长，抑制率达到（69．76±7．28）％（t=23．78，P〈0．01）；雷公藤红素联合顺铂用药组凋亡率为（47．75±5．63）％高于雷公藤红素组、顺铂组；酶联免疫吸附实验结果表明，雷公藤红素联合顺铂组Bax蛋白表达量明显高于单独用药组（t=10．59，P〈0．01），Bcl-2蛋白表达量、XIAP、NF-kB蛋白表达量明显低于单独用药组（t=35．27，P〈0．01）。结论雷公藤红素联合顺铂抑制体外C6胶质瘤细胞的增殖，可能是通过上调Bax蛋白的表达、降低抗凋亡蛋白Bcl、抑制XIAP及NF-kB蛋白的表达进而诱导肿瘤细胞凋亡。

Objective To clarify the inhibitory effect of Tripterine combined with Cisp]atin on C6 glioma cells and its apoptosis mechanism. Methods C6 glioma cells were treated with Tripterine, Cisplatin, or Tripterine combined with Cisplatin. CCK-8 assay was used to detect the growth inhibition rate in each group. Flow cytometry analysis was used to test the cell apoptosis rate. The expressions of apoptosis-related proteins including Bcl-2, Bax, XIAP, NF-kB were analyzed by ELISA. Results Compared with Tripterine- or Cisplatin-treated group, the inhibition ratio of cell growth in Tripterine and Cisplatin combination group was （69.76±7.28） %, which could significantly inhibit the growth of C6 glioma cells（t= 23.78, P〈0.01）. The apoptosis rate was significantly higher （47.75±5.63） %in combination group than in Tripterine or Cisplatin treated group. The results of ELISA showed that the expression of Bax was significantly higher and Bcl-2, XIAP, NF-κB were obviously lower in the combination group than in Tripterine or Cisplatin treated group （t= 35.27, P〈0.01）. Conclusion The combination of Tripterine and Cisplatin significantly increases the inhibition rate on C6 glioma cells through upregulating Bax and inhibiting Bcl, XIAP and NF-kB to induce the apoptosis of C6 glioma cells.