杂合抗菌肽Py-Sα真核表达载体的构建

Construction of Eukaryotic Expression Vector for Antibacterial Peptide Py-Sα Gene

本文根据抗菌肽Polyphemusin I和Spinigerinα的氨基酸序列,结合Pichia pastoris;偏爱密码子,人工设计合成杂合抗菌肽Py-Sα基因。按照正确的阅读框架将其定向克隆至真核表达载体p PICZαA上。经PCR及双酶切鉴定,所转化的宿主细胞中含有插入Py-Sα的重组质粒p PICZαA-Py-Sα,结果成功构建了杂合抗菌肽Py-Sα的真核表达载体。

According to the sequences of amino acid about Polyphemusin I and Spinigerin a antimicrobial peptides, combined with the partiality codon of Pichia pastoris, design and synthesis hybrid antimicrobial peptide of Py-Sa gene. In the correct reading frame which was cloned into the eukaryotie expression vector pPICZa A. After PCR and the double enzyme digestion to identify the transformation of Host cell containing the Py-Sa recombinant plasmid pPICZaA-Py-Sa. The results revealed the successful construction of hybrid antimicrobial peptide Py-Sa eukaryotie expression vector.