胃癌干细胞在肿瘤侵袭转移及对血管形成的影响

Gastric cancer stem cells in tumor invasion and metastasis and its influence on angiogenesis ability

背景:胃癌干细胞在新辅助化疗、常规治疗中发挥重要作用,与患者治疗后复发关系密切,但是该结论尚未得到证实。目的:观察胃癌干细胞在肿瘤侵袭转移中的作用及对血管形成能力的影响。方法:建立胃癌模型裸鼠,分离培养胃癌干细胞。将采集的胃癌干细胞分别进行细胞划痕实验、成环实验、细胞抑制率实验、细胞转移性实验及成瘤实验。结果与结论:(1)细胞形态:胃癌干细胞培养7 d后贴壁生长,贴壁细胞缺乏规律性,多为蝌蚪形;(2)划痕实验:划痕后0,24 h细胞划痕宽度差异有显著性意义(P<0.05);(3)成环实验:倒置显微镜观察显示,胃癌干细胞能形成环状;(4)细胞抑制率实验:奥沙利铂干预胃癌干细胞的半抑制浓度显著低于5-氟尿嘧啶(P<0.05);(5)胃癌肿瘤干细胞转移性实验:胃癌干细胞穿过基底膜细胞数显著高于细胞转移前(P<0.05);(6)成瘤实验:胃癌干细胞植入后胃癌组织质量显著重于普通胃癌裸鼠(P<0.05)。结果说明:胃癌干细胞在肿瘤侵袭、转移中发挥重要的作用,具备较强的血管形成能力。

BACKGROUND： Gastric cancer stem cells involved in the neoadjuvant chemotherapy and conventional treatment are closely associated with relapse of gastric cancer. However, this conclusion has not yet been confirmed.OBJECTIVE： To investigate the effects of gastric cancer stem cells in tumor invasion and metastasis and its effect on angiogenesis ability.METHODS： We prepared nude mouse models of gastric cancer to isolate and culture gastric cancer stem cells. Harvested gastric cancer stem cells were detected in cell scratch test, ring test, inhibition rate test,cell migration test and tumorigenicity test.RESULTS AND CONCLUSION： After 7 days of culture, the cells exhibited adherent growth but a lack of regularity that most cells were in a tadpole shape. In the cell scratch test, the scratch width was significantly different at 0 and 24 hours（P〈0.05）. Under an inverted microscope, the cells were found to form a ring in the ring test. The 50% inhibiting concentration of gastric cancer stem cells induced by oxaliplatin was significantly lower than that induced by 5-fluorouracil（P〈0.05）. The number of cells passing through the basilar membrane was significantly increased after cell migration（P〈0.05）. After implantation of gastric cancer stem cells, the gastric tissue quality was significantly higher than that in normal nude mice of gastric cancer（P〈0.05）. These findings indicate that gastric cancer stem cells responsible for tumor invasion and migration have stronger angiogenesis ability.