化疗前后乳腺癌患者血清中癌基因和抑癌基因表达水平研究

Expression levels of serum cancer gene and tumor suppressor gene in patients with breast cancer before and after chemotherapy

目的探讨化疗前、后乳腺癌患者血清中癌基因和抑癌基因的表达水平。方法将乳腺癌改良根治术术后首次接受化学治疗的乳腺癌女性患者74例作为试验组,同期体检乳腺正常的女性20例作为对照组;两组均清晨空腹采集静脉血,采用酶联免疫法和实时荧光定量法检测血清中癌基因C-myc和抑癌基因P53的蛋白及mRNA表达水平。结果化疗前试验组患者血清中C-myc的蛋白及mRNA表达水平均明显高于对照组、P53的蛋白及mRNA表达水平均明显低于对照组,两组差异均有统计学意义(P<0.01);化疗后试验组患者血清中C-myc、P53的蛋白及mRNA表达水平与对照组比较差异无统计学意义(P>0.05)。结论乳腺癌患者血清中癌基因C-myc表达水平的上调和抑癌基因P53表达水平的下调可能与乳腺癌的浸润和转移密切相关。

Objective To explore the expression levels of serum cancer gene and tumor suppressor gene in patients with breast cancer before and after chemotherapy. Methods Seventy- four female patients with breast cancer treated by chemotherapy for the first time after modified radical mastectomy were selected as experimental group,twenty women with normal breast after physical examination were selected as control group; early morning fasting venous blood samples were abstracted in the two groups,enzyme- linked immunosorbent assay（ ELISA） and real- time fluorescence quantitative method were used to detect expression levels of serum cancer gene C- myc and tumor suppressor gene P53 proteins and mRNA. Results The expression levels of serum C- myc protein and mRNA before chemotherapy in experimental group were statistically significantly higher than those in control group（ P〈0. 01）,while the expression levels of serum P53 protein and mRNA before chemotherapy in experimental group were statistically significantly lower than those in control group（ P〈0. 01）; after chemotherapy,there was no statistically significant difference in the expression levels of serum C- myc,P53 proteins and mRNA between experimental group and control group（ P〉0. 05）. Conclusion The up- regulation of serum C- myc expression and down- regulation of serum P53 expression in breast cancer patients may be closely related to invasion and metastasis of breast cancer.